MW:29,500
ExtinctionCoefficient:0.985

Salt-freelyophilizedsolid.

Reconstitution:Afterinitialcentrifugation,wesuggesttheadditionof50mMNaAcetate,pH5.5,with150mMNaCltotheoriginalvolume,followedbygentleswirlingand/orvortexingtoensureadequatehomogenization.

Storage:-20°C

BuypurifiedNativeHumanElastase(Leukocyte),humanneutrophilreagentdirectfromthemanufacturer.
Price$165.00/100ug-Samedayshipping.

Thisenzymedegradeselastin,collagen,andproteoglycanandhasbeenimplicatedinthedevelopmentofpulmonaryemphesemaandrheumatoidarthritis.

Ref:Baugh,R.J.andTravis,J.1976.Biochemistry.15,836;Bode,W.etAl.1986.EMBOJ.5(10),2453

Activity:20-22unitspermgprotein.OneunitisdefinedastheamountofenzymethathydrolyzesoneumoleofMeO-Suc-Ala-Ala-Pro-Val-pNAperminuteat25oC,pH7.5.

Purity:>=95%bySDS-PAGE

ProductCitations:
Mittendorf,ElizabethA.,GheathAlatrash,NaQiao,YunWu,PariyaSukhumalchandra,LisaS.StJohn,AnneV.Philipsetal."Breastcancercelluptakeoftheinflammatorymediatorneutrophilelastasetriggersananticanceradaptiveimmuneresponse." Cancerresearch 72,no.13(2012):3153-3162
vandenBerg,CarmenW.,etal."MechanismofNeutrophilDysfunction:NeutrophilSerineProteasesCleaveandInactivatetheC5aReceptor." TheJournalofImmunology 192.4(2014):1787-1795.
Edwards,J.Vincent,NicolettePrevost,KandanSethumadhavan,AbulUllah,andBrianCondon."Peptideconjugatedcellulosenanocrystalswithsensitivehumanneutrophilelastasesensoractivity." Cellulose (2013):1-13.

PreparedfromwholebloodshowntobenonreactiveforHBsAg,anti-HCV,anti-HBc,andnegativeforanti-HIV1&2byFDAapprovedtests.

AthensResearch&TechnologyproductsarelaboratoryreagentsandarenottobeadmiNISTeredtohumansorusedforanydrugpurpose.Forresearchuseonly.