
MW:25,000-29,000
ExtinctionCoefficient:1.28
Frozenin20mMMalonate,pH5.5,1mMEDTA,400mMNaCl.
Storage:-70°Corlower
BuyPurifiedNativeHumanCathepsinL(CatL),HumanLiver.
Price$160.00/25ug.BulkQtyAvailable.
Themostpowerfulofthelysosomalproteases.IthasahigherspecificactivitythancathepsinBandHinthedegradationofavarietyofphysiologicalproteinsubstrates.ThelevelofcathepsinLisastrongpredictorofrelapseandsurvivalfollowingtreatmentofaprimarybreasttumor.
References:
Mason,R.W.,Green,G.D.J.andBarrett,A.J.1985.Biochem.J.226,233.
Klijnetal.1998.J.Clin.Onc.16,1013.
Barrett,A.J.andKirschke,H.1981.MethodsEnzoymol.80,535.
Tchope,J.R.etal.1991.Biochem.Biophys.Acta.1076.149.
Activity:Greaterthan1unitpermgofprotein.OneunitisdefinedastheamountofenzymethathydrolyzesonemicromoleofZ-Phe-Arg-AFCperminuteat25oCusing400mMNaAcetate,pH5.5with4mMEDTAand8mMDTTastheactivationbufferinthepresenceofBrij.
ProductCitation:
Armstrong,Andrea,NiklasMattsson,HannaAppelqvist,CamillaJanefjord,LinneaSandin,LottaAgholme,BobOlssonetal."LysosomalNetworkProteinsasPotentialNovelCSFBioMarkersforAlzheimer’sDisease." Neuromolecularmedicine (2013):1-11
Miller,Bailey,AaronJ.Friedman,HyukjaeChoi,JamesHogan,J.AndrewMcCammon,VivianHook,andWilliamH.Gerwick."TheMarineCyanobacterialMetaboliteGallinamideAIsaPotentandSelectiveInhibitorofHumanCathepsinL." Journalofnaturalproducts (2013).
PreparedfromtissueshowntobenonreactiveforHBsAg,anti-HCV,anti-HBc,andnegativeforanti-HIV1&2byFDAapprovedtests.
AthensResearch&TechnologyproductsarelaboratoryreagentsandarenottobeadmiNISTeredtohumansorusedforanydrugpurpose.Forresearchorfurthermanufacturinguseonly.