MW:42,000
ExtinctionCoefficient:1.0

Lyophilizedfrom2mMNaphosphate,pH6.5.

Storage:-20°C

BuyPurifiedNativeHumanCathepsinD(CatD),HumanLiver.
Price$90.00/25ug.BulkQtyAvailable.

CathepsinDisanestrogen-regulatedproteinassociatedwithtissuebreakdown.LevelsofcathepsinDhavebeenpositivelycorrelatedwithrecurringbreastcancersofbothnode-negativeandnode-positivetypes.AdditionallycathepsinDhasbeenassociatedwithamyloidformationinAlzheimer"splaques.AscathepsinDactivityisincreasedbycigarettesmoke,theenzymemaycontributetolungtissuedamageinsmokers.
References:
Barrett,A.J.1977.ProteinasesinMammalianCellsandTissues.(A.Barrett,ed.)4,209.
Barrett,A.J.1970.Biochemistry.117,601.
Tandon,A.K.etal.1990.N.Engl.J.Med.322,297.
Cataldo,A.M.etal.1990.BrainRes.513,181.
Chang,J.C.etal.Am.Rev.Respir.Dis.140,958.

Activity:>=300unitspermgprotein.Oneunitisdefinedastheamountofenzymethatdigestshemoglobin-releasingpeptideswhicharesolublein10%TCA.ThereactionismeasuredbyanincreaseofanA280of1.0per60minutesat37°C.Substrate:aciddenaturedhemoglobin,pH1.8(0.2%inreactionmixture).Buffer:100mMformate,pH3.3.

Purity:>=95%bySDS-PAGE

ProductCitation:
Armstrong,Andrea,NiklasMattsson,HannaAppelqvist,CamillaJanefjord,LinneaSandin,LottaAgholme,BobOlssonetal."LysosomalNetworkProteinsasPotentialNovelCSFBioMarkersforAlzheimer’sDisease." Neuromolecularmedicine (2013):1-11
Appelqvist,Hanna,etal."Lysosome-mediatedapoptosisisassociatedwithcathepsinD-specificprocessingofbidatPhe24,Trp48,andPhe183."AnnalsofClinical&LaboratoryScience 42.3(2012):231-242.

PreparedfromtissueshowntobenonreactiveforHBsAg,anti-HCV,anti-HBc,andnegativeforanti-HIV1&2byFDAapprovedtests.

AthensResearch&TechnologyproductsarelaboratoryreagentsandarenottobeadmiNISTeredtohumansorusedforanydrugpurpose.Forresearchorfurthermanufacturinguseonly.