Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
HepG2/C3A Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)
HepG2/C3A Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HepG2/C3A Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-C3A0-1-05 | HepG2/C3A Cell Transfection Reagent (human hepatocellular carcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-C3A0-1-15 | HepG2/C3A Cell Transfection Reagent (human hepatocellular carcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
HepG2/C3A Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell)
HepG2/C3A Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HepG2/C3A Cells.
EZ Biosystems
Cell Line Information:
Designations: HepG2/C3A Depositors: Baylor College of Medicine Growth Properties:adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: liver Disease: hepatocellular carcinoma Cellular Products: alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; Tumorigenic: No Age: 15 years adolescent Gender:male Ethnicity: Caucasian Comments: C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion. Gluconeogenesis activity is strongly oxgen dependent. The cells have nitrogen metabolizing activity comparable to perfused rat livers.
The Transfection Reagent:
HepG2/C3A Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for HepG2/C3A Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in HepG2/C3A Cells.
- Extremely gentle to cells
- Efficient for plasmid DNA/siRNA co-transfection
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| HepG2/C3A Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies. Hepa 1-6 Cell Hep G2 Cell Hep 3B cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell CHO-K1 Cell SK-OV-3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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