Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
293T/17 Cell Avalanche™ Transfection Reagent
293T/17 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 293T/17 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293T/17 Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-293T-1-05 | 293T/17 Cell Avalanche™ Transfection Reagent | 0.5 ml | £399.00 | Quantity | Add to Order |
| EZT-293T-1-15 | 293T/17 Cell Avalanche™ Transfection Reagent | 1.5 ml | £699.00 | Quantity | Add to Order |
293T/17 Cell Avalanche™ Transfection Reagent
293T/17 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 293T/17 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293T/17 Cells.
EZ Biosystems
Cell line Information:
Designations:293T/17 [HEK 293T/17] Depositors: Rockefeller Univ. Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source: kidney Antigen Expression: SV40 T antigen [45408] Age: fetus Comments: The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.
The Transfection Reagent:
293T/17 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 293T/17 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293T/17 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into 293T/17 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate format
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| 293T/17 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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