Oligo Synthesis : CEPs
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N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1529-02 | N2-Amino-Modifier C6 dG | 0.25g | £880.00£836.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1529-90 | N2-Amino-Modifier C6 dG | 100µmoles | £384.00£364.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1529-95 | N2-Amino-Modifier C6 dG | 50µmoles | £192.00£182.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Glen Research
| N2-Amino-Modifier C6 dG |
Catalog Number: 10-1529-xx
Description: N2-Amino-Modifier C6 dG
| 5"-Dimethoxytrityl-N2-[6-(trifluoroacetylamino)-hex-1-yl]-2"-deoxyGuanosine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H60F3N8O8P | M.W.: 965.01 | F.W.: 428.38 |
Diluent: Anhydrous Acetonitrile |
| Coupling: N2-Amino-Modifier C6 dG reacts in a manner identical to normal phosphoramidites. To prevent side reactions, synthesize using acetyl-protected dC. See Deprotection for further details. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. The TFA protecting group is removed during standard ammonium hydroxide deprotection. A minor side reaction during ammonia deprotection can lead to irreversibly capping 2-5% of the amine. This could be significant if multiple additions of the modifier are made. To prevent the reaction, synthesize using acetyl-protected dC and deprotect in 30% ammonia/40% methylamine 1:1 (AMA) at 65°C for 10 minutes. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 2-3 days SEQUENCE MODIFIERSSequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide. |
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N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Glen Research
Material Safety Data Sheet
| N2-amino-dG.pdf: N2-Amino-Modifier C6 dG - A new dG Amino-Modifier with improved hybridization characteristics |
| Glen Report 22.1: New Product - A dG Amino-Modifier with improved hybridization characteristics |
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N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Glen Research
Technical Notes
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N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1529-02 | 0.25grams | .25grams | 2.59 | 73 | 43.8 | 27.38 | 19.91 | 14.6 | 3.65 |
| 10-1529-90 | 100µmoles | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1529-95 | 50µmoles | .048grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1529-02 | 0.25grams | .25grams | 3.87 | .07 | 71 | 44.38 | 32.27 | 4.44 | |
| 10-1529-90 | 100µmoles | .097grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1529-95 | 50µmoles | .048grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1529-02 | 0.25grams | .25grams | 3.87 | .07 | 72.6 | 45.38 | 33 | ||
| 10-1529-90 | 100µmoles | .097grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1529-95 | 50µmoles | .048grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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