Oligo Synthesis : CEPs
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DesthiobiotinTEG Phosphoramidite
DesthiobiotinTEG Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1952-02 | DesthiobiotinTEG Phosphoramidite | 0.25g | £620.00£589.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1952-90 | DesthiobiotinTEG Phosphoramidite | 100µmoles | £268.00£254.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1952-95 | DesthiobiotinTEG Phosphoramidite | 50µmoles | £148.00£140.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
DesthiobiotinTEG Phosphoramidite
DesthiobiotinTEG Phosphoramidite
Glen Research
| DesthiobiotinTEG Phosphoramidite |
Catalog Number: 10-1952-xx
Description: DesthiobiotinTEG Phosphoramidite
| 1-Dimethoxytrityloxy-3-O-(N-desthiobiotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C52H78N5O11P | M.W.: 980.19 | F.W.: 539.56 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 12-15 minute coupling time.To maintain label yield, carry out the synthesis DMT-On. |
| Deprotection: No changes needed from standard method recommended by synthesier manufacturer.To maintain label yield, remove the 5"-DMT after ammonium hydroxide deprotection. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Biotin-dT can replace dT residues within the oligonucleotidesequence. 5’-Biotin phosphoramidite can be added ONLY ONCE to the 5’-terminus ofan oligonucleotide. However, the DMT group on the biotin can be used in RPcartridge and HPLC purification techniques. PC Biotin is a photocleavable5’-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG aredesigned for the direct synthesis of oligonucleotides containing biotin at the3’ terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding tobiotin-binding proteins such as streptavidin. This biotin analogue is lackingthe sulfur group from the molecule and has a dissociation constant (Kd) severalorders of magnitude less than biotin/streptavidin. As a result, biomoleculescontaining desthiobiotin are dissociated from streptavidin simply in thepresence of buffered solutions of biotin. We offer desthiobiotinTEGphosphoramidite and the corresponding CPG.
ABI-style vials and columns are supplied unless otherwise requested.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
DesthiobiotinTEG Phosphoramidite
DesthiobiotinTEG Phosphoramidite
Glen Research
MSDS
Glen Report 16.2: STILL MORE NEW PRODUCTS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
DesthiobiotinTEG Phosphoramidite
DesthiobiotinTEG Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1952-02 | 0.25grams | .25grams | 2.55 | 71.67 | 43 | 26.88 | 19.55 | 14.33 | 3.58 |
| 10-1952-90 | 100µmoles | .098grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1952-95 | 50µmoles | .049grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1952-02 | 0.25grams | .25grams | 3.81 | .07 | 69.8 | 43.63 | 31.73 | 4.36 | |
| 10-1952-90 | 100µmoles | .098grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1952-95 | 50µmoles | .049grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1952-02 | 0.25grams | .25grams | 3.81 | .07 | 71.4 | 44.63 | 32.45 | ||
| 10-1952-90 | 100µmoles | .098grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1952-95 | 50µmoles | .049grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
