Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Dabcyl-dT
Dabcyl-dT
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1058-02 | Dabcyl-dT | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1058-90 | Dabcyl-dT | 100µmoles | £260.00£247.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1058-95 | Dabcyl-dT | 50µmoles | £144.00£136.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Dabcyl-dT
Dabcyl-dT
Glen Research
| Dabcyl-dT |
Catalog Number: 10-1058-xx
Description: Dabcyl-dT
| 5"-Dimethoxytrityloxy-5-[(N-4"-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2"-deoxyUridine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C63H76N9O10P | M.W.: 1150.32 | F.W.: 709.7 |
Diluent: 10% THF in Anhydrous Acetonitrile |
| Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1).A 6 minute coupling time is recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescencequenched in solution unless it is hybridized to the target sequence.Consequently, the design of a molecular beacon requires a fluorophore to be inone part of the sequence and the quencher molecule to be in another, with bothmolecules being separated from the oligonucleotide by a hydrocarbon spacer. TheDabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and3’-Dabcyl CPG are used to prepare probes with the quencher blocking the3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminusand Dabcyl-dT places it within the sequence, leaving the 3’-terminus availablefor polymerase extension.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Dabcyl-dT
Dabcyl-dT
Glen Research
MSDS
| Glen Report 11.1: NEW FLUORESCENT REAGENTS - TAMRA-dT, DABCYL-dT |
| Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Dabcyl-dT
Dabcyl-dT
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1058 | Dabcyl-dT | 476 nm | 32.0 (L/mmole-cm) | 29.1 (L/mmole-cm) |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 2.17 | 59 | 35.4 | 22.13 | 16.09 | 11.8 | 2.95 |
| 10-1058-90 | 100µmoles | .115grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1058-95 | 50µmoles | .058grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 3.24 | .07 | 58.4 | 36.5 | 26.55 | 3.65 | |
| 10-1058-90 | 100µmoles | .115grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1058-95 | 50µmoles | .058grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 3.24 | .07 | 60 | 37.5 | 27.27 | ||
| 10-1058-90 | 100µmoles | .115grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1058-95 | 50µmoles | .058grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
