Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
CleanAmp™-Pac-dA-CE Phosphoramidite
CleanAmp™-Pac-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1440-02 | CleanAmp™-Pac-dA-CE Phosphoramidite | 0.25g | £192.00£182.40Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1440-05 | CleanAmp™-Pac-dA-CE Phosphoramidite | 0.5g | £384.00£364.80Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1440-90 | CleanAmp™-Pac-dA-CE Phosphoramidite | 100µmoles | £80.00£76.00Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
CleanAmp™-Pac-dA-CE Phosphoramidite
CleanAmp™-Pac-dA-CE Phosphoramidite
Glen Research
| CleanAmp™-Pac-dA-CE Phosphoramidite |
Catalog Number: 10-1440-xx
Description: CleanAmp™-Pac-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-phenoxyacetyl-2"-deoxyAdenosine,3"-[(4-oxo-tetradecyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C59H77N6O9P | M.W.: 1045.25 | F.W.: FW: 523.56 (triester) |
Diluent: Anhydrous Acetonitrile |
| Coupling: 10 minute coupling time.CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix. |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature.See Glen Report 21.2 for details. (www.glenresearch.com/GlenReports/GR21-2.pdf) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
CleanAmp™ Primers
CleanAmp™ Primers offer an alternative to other Hot Start technologies andallow greater control of primer hybridization and extension during PCR. It hasbeen demonstrated that CleanAmp™ Primers outperform other technologies inmultiple applications. Indeed, over a broad range of applications, CleanAmp™Primers reduce or eliminate off-target amplification. Greater amplicon yield isalso achieved, due to improvement in specificity and sensitivity. By usingeither the slow-releasing Precision primers with two CleanAmp™ phosphotriesterlinkages or the faster-releasing Turbo Primers with a single CleanAmp™phosphotriester linkage, the rate of formation of unmodified primer can becontrolled to suit reaction needs. A table to aid in the selection of Turbo andPrecision Primers for specific applications is shown below.
| Turbo Primers | Precision Primers |
| Fast cycling | Standard cycling |
| Multiplex PCR | One-step reverse-transcription PCR |
| Improves amplicon yield | Improved specificity and limit ofdetection |
| Reduces mis-priming/ primer dimer formation | Greatest reduction in mis-priming/primer dimerformation |
Synthesis of CleanAmp™ Primers requires the use of UltraMild Chemistry.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
CleanAmp™-Pac-dA-CE Phosphoramidite
CleanAmp™-Pac-dA-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 21.1: HOT START PCR UPDATE: CLEANAMP™ PRIMERS |
| Glen Report 21.1: PROCEDURE FOR SYNTHESIS OF CLEANAMP™ PRIMERS |
| Glen Report 21.2: Purification of CleanAmp™ DNA Oligonucleotides (DMT-ON) |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
CleanAmp™-Pac-dA-CE Phosphoramidite
CleanAmp™-Pac-dA-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1470-02 | 0.25grams | .25grams | 2.77 | 79 | 47.4 | 29.63 | 21.55 | 15.8 | 3.95 |
| 10-1470-05 | 0.5grams | .5grams | 5.54 | 171.33 | 102.8 | 64.25 | 46.73 | 34.27 | 8.57 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1470-02 | 0.25grams | .25grams | 4.14 | .07 | 76.4 | 47.75 | 34.73 | 4.78 | |
| 10-1470-05 | 0.5grams | .5grams | 8.27 | .07 | 159 | 99.38 | 72.27 | 9.94 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1470-02 | 0.25grams | .25grams | 4.14 | .07 | 78 | 48.75 | 35.45 | ||
| 10-1470-05 | 0.5grams | .5grams | 8.27 | .07 | 160.6 | 100.38 | 73 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
