Oligo Synthesis : CEPs
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Cis-syn Thymine Dimer Phosphoramidite
Cis-syn Thymine Dimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 11-1330-02 | Cis-syn Thymine Dimer Phosphoramidite | 0.25g | £10,000.00£7,752.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 11-1330-90 | Cis-syn Thymine Dimer Phosphoramidite | 100µmoles | £4,112.00£3,192.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 11-1330-95 | Cis-syn Thymine Dimer Phosphoramidite | 50µmoles | £2,064.00£1,596.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Cis-syn Thymine Dimer Phosphoramidite
Cis-syn Thymine Dimer Phosphoramidite
Glen Research
| Cis-syn Thymine Dimer Phosphoramidite |
Catalog Number: 11-1330-xx
Description: Cis-syn Thymine Dimer Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxy-5,6-dihydro-Thymidine-(cis-syn-cyclobutanyl)-5"->3"-(methyl-phosphoryl)-2"-deoxy-5,6-dihydro-Thymidine-3"-[methyl-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C49H63N5O15P2 | M.W.: 1024.01 | F.W.: 608.39 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 10 minute coupling recommended. |
| Deprotection: 1.Remove the methyl phosphate groups by treating the support with thiophenol/triethylamine/THF (1:2:2) v/v/v for 45 minutes at room temperature.Wash the support with THF 1X, methanol 5X, acetonitrile 3X and dry with air or argon.2.Cleave and deprotect the oligo in the dark with ammonium hydroxide at room temperature under conditions appropriate to remove the protecting groups on the nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
STRUCTURE/ACTIVITY RELATIONSHIP
The following products are used to investigate the effect on theactivity of an oligonucleotide when key structural elements are changed. The7-deaza purine monomers lack groups critical for hydrogen bonding.7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G andhave similar electron density. Their presence in oligos is slightly stabilizingrelative to A and G. Unlike G, PPG does not lead to aggregation and G-richoligos can be easily prepared and isolated. 5’-Fluorescein oligos with PPG atthe 5’-terminus are much less quenched than the equivalent G oligos. As a purineanalogue of Thymidine, 7-deaza-2’-deoxyXanthosine (7-deaza-dX) promises to haveinteresting effects on DNA structure of triplexes. 7-Deaza-dX also forms anon-standard base pair with a 2,4-diaminopyrimidine nucleoside analogue.Standard nucleobases have an unshared pair of electrons that project into the minor groove of duplex DNA. Enzymes that interact with DNA,polymerases, reverse transcriptases, restriction enzymes, etc., may use ahydrogen bond donating group to contact the hydrogen bond acceptor in the minorgroove. 3-Deaza-2’-deoxyadenosine is very interesting in that it maintains theability for regular Watson-Crick hydrogen bonding to T but is lacking theelectron pair at the 3-position normally provided by N3.
One of the major sources of DNA damage in all organisms is the UV component ofsunlight. The predominant reaction induced by UV light on DNA is dimerization ofadjacent pyrimidine bases leading to cyclobutane dimers (CPDs). The dimersformed in the most significant quantity are the cis-syn cyclobutane dimer of twothymine bases. Although formed routinely, these dimer products are efficientlyexcised and repaired enzymatically (nucleotide excision repair) or thedimerization is reversed by photolase enzymes. These lesions have been connectedto the formation of squamous cell carcinomas. In addition, humans who lackability to repair CPD lesions with high efficiency may be geneticallypredisposed to Xeroderma Pigmentosa (XP), a disease characterized by extremesensitivity to sunlight and high frequency of skin cancer. Polymerasesencountering unrepaired CPD lesions are quite error-prone, leading to incorrectbase insertions and subsequent mutations.
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Cis-syn Thymine Dimer Phosphoramidite
Cis-syn Thymine Dimer Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 16.2: Thymine dimers - DNA lesions induced by sunlight cis-syn thymine dimer phosphoramidite now available
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Cis-syn Thymine Dimer Phosphoramidite
Cis-syn Thymine Dimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 11-1330-02 | 0.25grams | .25grams | 2.44 | 68 | 40.8 | 25.5 | 18.55 | 13.6 | 3.4 |
| 11-1330-90 | 100µmoles | .102grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 11-1330-95 | 50µmoles | .051grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 11-1330-02 | 0.25grams | .25grams | 3.64 | .07 | 66.4 | 41.5 | 30.18 | 4.15 | |
| 11-1330-90 | 100µmoles | .102grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 11-1330-95 | 50µmoles | .051grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 11-1330-02 | 0.25grams | .25grams | 3.64 | .07 | 68 | 42.5 | 30.91 | ||
| 11-1330-90 | 100µmoles | .102grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 11-1330-95 | 50µmoles | .051grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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