Oligo Synthesis : CEPs
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Cholesteryl-TEG Phosphoramidite
Cholesteryl-TEG Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1975-02 | Cholesteryl-TEG Phosphoramidite | 0.25g | £436.00£414.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1975-90 | Cholesteryl-TEG Phosphoramidite | 100µmoles | £212.00£201.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1975-95 | Cholesteryl-TEG Phosphoramidite | 50µmoles | £112.00£106.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Cholesteryl-TEG Phosphoramidite
Cholesteryl-TEG Phosphoramidite
Glen Research
| Cholesteryl-TEG Phosphoramidite |
Catalog Number: 10-1975-xx
Description: Cholesteryl-TEG Phosphoramidite
| 1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol-glyceryl-2-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite | ||
| Formula: C70H106N3O11P | M.W.: 1196.60 | F.W.: 755.97 |
Diluent: 10% THF in Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 15 minute coupling time.To maintain label yield, carry out the synthesis DMT-on. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.To maintain label yield,remove the 5"-DMT after ammonium hydroxide deprotection. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
CHOLESTEROL LABELLING
Potential therapeutic oligonucleotides must permeate the cellmembrane for optimal activity. The addition of lipophilic groups to anoligonucleotide would be expected to enhance activity. The use of cholesteryloligos and the consequent improvement in activity has been described. We havedesigned our Cholesteryl Phosphoramidite with our branched triethyleneglycol(TEG) spacer for maximum solubility in acetonitrile as well as for applicationsrequiring multiple labels.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Cholesteryl-TEG Phosphoramidite
Cholesteryl-TEG Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA |
| Glen Report 6.1: NEW LABELLING REAGENTS |
| Glen Report 20.1: New Product |
| Glen Report 24.1: New Product - 5"-Stearyl Phosphoramidite - An Alternative Lipophilic Carrier |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Cholesteryl-TEG Phosphoramidite
Cholesteryl-TEG Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 2.09 | 56.33 | 33.8 | 21.13 | 15.36 | 11.27 | 2.82 |
| 10-1975-90 | 100µmoles | .12grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1975-95 | 50µmoles | .06grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 3.12 | .07 | 56 | 35 | 25.45 | 3.5 | |
| 10-1975-90 | 100µmoles | .12grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1975-95 | 50µmoles | .06grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 3.12 | .07 | 57.6 | 36 | 26.18 | ||
| 10-1975-90 | 100µmoles | .12grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1975-95 | 50µmoles | .06grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
