Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Carboxy-dT
Carboxy-dT
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1035-02 | Carboxy-dT | 0.25g | £288.00£273.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1035-90 | Carboxy-dT | 100µmoles | £144.00£136.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Carboxy-dT
Carboxy-dT
Glen Research
| Carboxy-dT |
Catalog Number: 10-1035-xx
Description: Carboxy-dT
| 5"-Dimethoxytrityl-5-[3-methyl-acrylate]-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H51N4O10P | M.W.: 814.88 | F.W.: 360.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.Note: NaOH is not compatible with dmf protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 2-3 days SEQUENCE MODIFIERSSequence Modifiers are designed for use in automated synthesis.The carboxy-dT is hydrolyzed during deprotection and can be coupled directly toa molecule containing a primary amino group by a standard peptide coupling orvia the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA,Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can beadded in place of a dA, dC, dG and dT residue, respectively, duringoligonucleotide synthesis. Corresponding Amino-Modifier supports can replacetheir respective deoxynucleoside supports. After deprotection, the primary amineon the C6 analogues is separated from the oligonucleotide by a spacer arm with atotal of 7 -10 atoms and can be labelled or attached to an enzyme. The C2analogue is more suitable for the attachment of molecules designed to react withthe oligonucleotide. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Carboxy-dT
Carboxy-dT
Glen Research
Material Safety Data Sheet
Glen Report 6.1: DEOXYURIDINE DERIVATIVES FOR INTERNAL MODIFICATION
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Carboxy-dT
Carboxy-dT
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1035 | Carboxy-dT | 297 | 16.1 | 261 | 14.7 | 14.7 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1035-02 | 0.25grams | .25grams | 3.07 | 89 | 53.4 | 33.38 | 24.27 | 17.8 | 4.45 |
| 10-1035-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1035-02 | 0.25grams | .25grams | 4.58 | .07 | 85.2 | 53.25 | 38.73 | 5.33 | |
| 10-1035-90 | 100µmoles | .081grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1035-02 | 0.25grams | .25grams | 4.58 | .07 | 86.8 | 54.25 | 39.45 | ||
| 10-1035-90 | 100µmoles | .081grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
