Oligo Synthesis : CEPs
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CAG Trimer Phosphoramidite
CAG Trimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 13-1102-90 | CAG Trimer Phosphoramidite | 100µmoles | £680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 13-1102-95 | CAG Trimer Phosphoramidite | 50µmoles | £340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
CAG Trimer Phosphoramidite
CAG Trimer Phosphoramidite
Glen Research
| CAG Trimer Phosphoramidite |
Catalog Number: 13-1102-xx
Description: CAG Trimer Phosphoramidite
| 5"-Dimethoxytrityl-N4-benzoyl-2"-deoxyCytidine-3"->5"-o-chlorophenyl-phosphoryl-N6-benzoyl-2"-deoxyAdenosine-3"->5"-o-chlorophenyl-phosphoryl-N2-isobutyryl-2"-deoxyGuanosine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C89H92Cl2N15O21P3 | M.W.: 1869.5 | |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling:15 minute coupling time recommended. |
| Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Trimer Phosphoramidites
In principle, the simplest approach for oligonucleotide-directedmutagenesis would be the use of trimer phosphoramidites. Of the 64 possiblecombinations of codons, only 20 codons would be required to cover the 20 aminoacids, although, in practice, several codons will likely be duplicated dependingon the organism. Our trimers use the protection scheme described1-3 by Kayushin et al. There is a concern that the sequence of the trimers has to beverified. For example, CAT coding for histidine, has to be differentiated fromTAC, coding for tyrosine. These two trimers have virtually identicallipophilicity and their identity cannot be clearly confirmed by HPLC. Thisproblem has been solved4 using HPLC electrospray mass spectrometricanalysis of the trimers, which provides data confirming molecular weight andsequence.
In Table 1, the trimers, their coding amino acid and theirreaction factor (RF) are listed. The reaction factor is critical since thetrimers will likely be mixed and they have differing reactivity in the couplingreaction. RF for AAC is 1.0 and for TAC is 1.6. Therefore, 1.6 equivalents ofTAC are needed for every 1.0 equivalent of AAC for equal coupling. Mixtures caneasily be made using equimolar solutions or the molecular weight of each trimerhas to be used to generate the appropriate weights of each trimer to use ifmixing by weight. An example of the preparation of a mixture of all 20 trimersis shown in the right column of Table 1 and completed in the footnotes.
All of the trimers are now available individually so that researchers can prepare custommixtures. A mixture of all 20 trimers designed to produce equal coupling of all20 is also available. If you require custom production of a specific mixture,please e-mail support@glenresearch.com for aquotation and projected delivery.
Example of Preparation of Trimer Mixture
Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer fromthe right column. Dissolve the trimer mix in dichloromethane (highest gradepossible; acid-free). Evaporate to dryness to produce a homogenous mixture ofall 20 trimers.
Example of Preparation of Trimer Mixture for the Synthesizer
Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) ofthe trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v toproduce a 0.10N solution of trimers, ready for use in a synthesizer.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
CAG Trimer Phosphoramidite
CAG Trimer Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION |
| Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
CAG Trimer Phosphoramidite
CAG Trimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 13-1102-90 | 100µmoles | .187grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 13-1102-95 | 50µmoles | .093grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 13-1102-90 | 100µmoles | .187grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 13-1102-95 | 50µmoles | .093grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 13-1102-90 | 100µmoles | .187grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 13-1102-95 | 50µmoles | .093grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
