

Oligo Synthesis : CEPs
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Br-U-CE Phosphoramidite
Br-U-CE Phosphoramidite
Glen Research
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
10-3090-02 | Br-U-CE Phosphoramidite | 0.25g | £380.00£361.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-3090-90 | Br-U-CE Phosphoramidite | 100µmoles | £156.00£148.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-3090-95 | Br-U-CE Phosphoramidite | 50µmoles | £78.40£74.48Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Br-U-CE Phosphoramidite
Br-U-CE Phosphoramidite
Glen Research
Br-U-CE Phosphoramidite |
Catalog Number: 10-3090-xx
Description: Br-U-CE Phosphoramidite
5"-Dimethoxytrityl-5-bromo-Uridine,2"-O-TBDMS,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
Formula: C45H60N4O9BrPSi | M.W.: 939.96 | F.W.: 385.06 |
Diluent: Anhydrous Acetonitrile |
Coupling: 12 minutes |
Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases.Complete the 2" deprotection using standard RNA protocols. Technical Bulletin |
Storage: Freezer storage, -10 to -30°C, dry |
Stability in Solution: 24 hours |
minor rna phosphoramidites (TBDMS PROTECTED)
Inosine and 5-Methyl-Uridine are useful for analyzing RNAstructure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine havebeen used for crystallography studies and cross-linking experiments.6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, aswell as in RNA-protein interactions. The removal of the silyl protecting groupwithout interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride(TBAF) leads to degradation of the thio-nucleotide analogue and should not beused. 2-Aminopurine riboside is useful for analyzing RNA structure and activityrelationships, for example, in ribozyme studies.
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Br-U-CE Phosphoramidite
Br-U-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 7.1: PROPYNYL-2"-OMe-RNA, Br-U, I-U |
Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Br-U-CE Phosphoramidite
Br-U-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
ABI 392/394 | |||||||||
Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
Approximate Number of Additions | |||||||||
10-3090-02 | 0.25grams | .25grams | 2.66 | 75.33 | 45.2 | 28.25 | 20.55 | 15.07 | 3.77 |
10-3090-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-3090-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
Expedite | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
Approximate Number of Additions | |||||||||
10-3090-02 | 0.25grams | .25grams | 3.97 | .07 | N/A | N/A | 15.21 | N/A | |
10-3090-90 | 100µmoles | .094grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
10-3090-95 | 50µmoles | .047grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
Beckman | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
Approximate Number of Additions | |||||||||
10-3090-02 | 0.25grams | .25grams | 3.97 | .07 | N/A | 12.03 | 11.3 | ||
10-3090-90 | 100µmoles | .094grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
10-3090-95 | 50µmoles | .047grams | .75 | .07 | N/A | 1.65 | 1.55 |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200