Oligo Synthesis : CEPs
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ATG Trimer Phosphoramidite
ATG Trimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 13-1032-90 | ATG Trimer Phosphoramidite | 100µmoles | £680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 13-1032-95 | ATG Trimer Phosphoramidite | 50µmoles | £340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
ATG Trimer Phosphoramidite
ATG Trimer Phosphoramidite
Glen Research
| ATG Trimer Phosphoramidite |
Catalog Number: 10-1032-xx
Description: O4-Me-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-O4-methyl-2"-deoxyThymidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H51N4O8P | M.W.: 758.85 | F.W.: 318.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer.Monomers that allow for UltraMILD deprotection are recommended(Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.)To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection:If UltraMild Monomers were used:0.05M Potassium Carbonate in Methanol, 4hrs at Room Temp.DMT-Off Synthesis: Add 6uL acetic acid per mL 0.05M potassium carbonate in methanol.Dry under vacuum. Desalt per standard procedures.DMT-On Synthesis: Use a Glen-Pak Purification Cartridge (60-5100-XX/60-5200-XX) to purify and desalt the oligonucleotide.Dilute the 0.05M potassium carbonate in methanol with 100mg/mL Sodium Chloride to a final concentration of 1:4(v/v).Load onto the prepped Glen-Pak Cartridge and purify as normal. If Standard Monomers were used: 1) Prepare a solution of 10% DBU in anhydrous methanol. DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) is available from Aldrich (139009-25G) as is anhydrous methanol (322415-100ML). 2) Transfer the CPG to a clean, dry glass vial and add 1mL of the 10% DBU solution. 3) Seal the vial and leave for 5 days in the dark at room temperature. 4) Transfer the contents to a micro centrifuge tube and reduce under vacuum to a small volume. 5) Add 1mL of 10mM aqueous sodium hydroxide solution and desalt or purify the oligonucleotide using standard procedures. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
MUTAGENESIS
Cellular polynucleotides are alkylated by endogenous components, such asS-adenosylmethionine, or after reacting with two general classes ofenvironmental and laboratory chemicals. SN1 chemical agents includealkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, andthe non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2chemical agents such as methyl methanesulfonate and dimethyl sulfate reactprimarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 ofcytosine. To avoid chain branching during synthesis when using DCI as activator,N6-Me-dA is offered with acetyl protection.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
ATG Trimer Phosphoramidite
ATG Trimer Phosphoramidite
Glen Research
MSDS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
ATG Trimer Phosphoramidite
ATG Trimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1032-02 | 0.25grams | .25grams | 3.29 | 96.33 | 57.8 | 36.13 | 26.27 | 19.27 | 4.82 |
| 10-1032-90 | 100µmoles | .076grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1032-02 | 0.25grams | .25grams | 4.92 | .07 | 92 | 57.5 | 41.82 | 5.75 | |
| 10-1032-90 | 100µmoles | .076grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1032-02 | 0.25grams | .25grams | 4.92 | .07 | 93.6 | 58.5 | 42.55 | ||
| 10-1032-90 | 100µmoles | .076grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
