Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
ATC Trimer Phosphoramidite
ATC Trimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 13-1031-90 | ATC Trimer Phosphoramidite | 100µmoles | £680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 13-1031-95 | ATC Trimer Phosphoramidite | 50µmoles | £340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
ATC Trimer Phosphoramidite
ATC Trimer Phosphoramidite
Glen Research
| ATC Trimer Phosphoramidite |
Catalog Number: 10-1031-xx
Description: 5"-OMe-dT-CE Phosphoramidite
| 5"-O-methyl-2"-deoxyThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C20H33N4O6P | M.W.: 456.48 | F.W.: 318.22 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.(Note: this product is a 5"-terminator.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
chainterminators
In situations where ligation must be blocked at the 5’ terminus,5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-Tcan control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may beused for attaching a peptide or a PNA sequence. To avoid polymerase extension atthe 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have provedto be effective. 2’,3’- Phosphoramidites are designed to be used with the5’-phosphoramidites and supports. Since these phosphoramidites have no DMTgroup, they are not compatible with purification by the DMT-on technique. Ionexchange HPLC or PAGE should be used to purify these dideoxy terminated oligosto ensure that shorter sequences (containing 3’-OH) groups are removed.(3’-Termination can also be effected using a 3’-3’ linkage formed using5’-supports, or 3’-spacer C3 CPG.)
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ATC Trimer Phosphoramidite
ATC Trimer Phosphoramidite
Glen Research
MSDS
Glen Report 20.1: Technical Brief - New application for 5Õ-OMe-dT phosphoramidite
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
ATC Trimer Phosphoramidite
ATC Trimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1031-02 | 0.25grams | .25grams | 5.48 | 169.33 | 101.6 | 63.5 | 46.18 | 33.87 | 8.47 |
| 10-1031-90 | 100µmoles | .046grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1031-02 | 0.25grams | .25grams | 8.17 | .07 | 157 | 98.13 | 71.36 | 9.81 | |
| 10-1031-90 | 100µmoles | .046grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1031-02 | 0.25grams | .25grams | 8.17 | .07 | 158.6 | 99.13 | 72.09 | ||
| 10-1031-90 | 100µmoles | .046grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
