Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
AP-dC-CE Phosphoramidite
AP-dC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1097-02 | AP-dC-CE Phosphoramidite | 0.25g | £940.00£893.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1097-90 | AP-dC-CE Phosphoramidite | 100µmoles | £368.00£349.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1097-95 | AP-dC-CE Phosphoramidite | 50µmoles | £184.00£174.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
AP-dC-CE Phosphoramidite
AP-dC-CE Phosphoramidite
Glen Research
| AP-dC-CE Phosphoramidite |
Catalog Number: 10-1097-xx
Description: AP-dC-CE Phosphoramidite
| 1-[5"-O-(4,4"-Dimethoxytrityl)-?-D-2"-deoxyribofuranosyl]-9-(2-trifluoroacetamidoethoxy)-1,3-diaza-2-oxophenoxazine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite | ||
| Formula: C49H54F3N6O10P | M.W.: 974.97 | F.W.: 438.33 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection:AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 10 minutes at 65 °C is recommended.Note to prevent any modification of dC by Methylamine, use Ac-dC (10-1015-xx) during synthesis. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
Please Note: This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. ("Isis"). Purchase of this product includes a limited license to use this product solely for internal research. This license specifically excludes (and you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Isis. Neither this product nor any product created through its use may be used in human clinical trials. C-5 propyne derivatives and G-ClampSubstitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU(pdU) for dT are effective strategies to enhance base pairing. Using these basesubstitutions, duplex stability and melting temperatures are raised by thefollowing amounts: C-5 propynyl-C 2.8° per substitution; C-5 propynyl-U 1.7° persubstitution. AP-dC (G-clamp) substitutes for dC and is another very importantmodified nucleoside that enhances hybridization by 7.5° per substitution. Theability of these modified bases to enhance binding while maintaining specificityhas proven useful in antisense research and in the synthesis of high affinityprobes. AP-dC is also a fluorescent nucleoside and should find uses in DNAstructural research. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AP-dC-CE Phosphoramidite
AP-dC-CE Phosphoramidite
Glen Research
MSDS
| Glen Report 18.1: Fluorous Affinity Purification of Oligonucleotides |
| Glen Report 19.2: AP-dC is compatible with PCR and is Highly Fluorescent |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AP-dC-CE Phosphoramidite
AP-dC-CE Phosphoramidite
Glen Research
C-5 Propyne and AP-dC Phosphoramidites
This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. (Isis). Purchase of this product includes a limited licence to use this product solely for internal research. This licence specifically excludes (an you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreigh counterpart), (c) resale (including sale of any products or services that incorporate this product), or (d) gene functionalisation activities (including products or services that incorporate data derived from gene functionalisation activities) if such activities have commercial application, any and all of which require a separate licence from Isis. Neither this product nor any product created through its use may be used in human clinical trials.
A simple agreement must be signed before end-users and custom oligo services amy purchase these products for use as defined above.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AP-dC-CE Phosphoramidite
AP-dC-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1097 | 362nm | 10.5 | 10.9 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1097-02 | 0.25grams | .25grams | 2.56 | 72 | 43.2 | 27 | 19.64 | 14.4 | 3.6 |
| 10-1097-90 | 100µmoles | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1097-95 | 50µmoles | .049grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1097-02 | 0.25grams | .25grams | 3.83 | .07 | 70.2 | 43.88 | 31.91 | 4.39 | |
| 10-1097-90 | 100µmoles | .097grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1097-95 | 50µmoles | .049grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1097-02 | 0.25grams | .25grams | 3.83 | .07 | 71.8 | 44.88 | 32.64 | ||
| 10-1097-90 | 100µmoles | .097grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1097-95 | 50µmoles | .049grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
