Oligo Synthesis : CEPs
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Alkyne-Modifier Serinol Phosphoramidite
Alkyne-Modifier Serinol Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1992-02 | Alkyne-Modifier Serinol Phosphoramidite | 0.25 g | £460.00£437.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1992-90 | Alkyne-Modifier Serinol Phosphoramidite | 100 µmole | £148.00£140.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1992-95 | Alkyne-Modifier Serinol Phosphoramidite | 50 µmole | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Alkyne-Modifier Serinol Phosphoramidite
Alkyne-Modifier Serinol Phosphoramidite
Glen Research
Alkyne-Modifier Serinol Phosphoramidite
Catalog Number: 10-1992-xx
Description: Alkyne-Modifier Serinol Phosphoramidite
| 3-Dimethoxytrityloxy-2-(3-(5-hexynamido)propanamido)propyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H55N4O7P | M.W.: 758.88 | F.W.: 334.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. |
| Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65 °C, 17 hours at room temperature or AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65 °C |
| Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
CONJUGATION USING CLICK CHEMISTRY (CONT.)
Oligonucleotides prepared using 5"-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3"-end or the 5"-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5"-terminus, 5"-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5"-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5" terminus or within the sequence, and a synthesis support for labelling the 3" terminus of oligonucleotides with an alkyne group. A 5"-iodo-modified oligonucleotide (prepared using 5"-Iodo-dT) can be quantitatively converted to the corresponding 5"-azide.
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Alkyne-Modifier Serinol Phosphoramidite
Alkyne-Modifier Serinol Phosphoramidite
Glen Research
Material safety Data Sheet
Glen Report 24.2: New Product - Alkyne-Modifier Serinol Phosphoramidite
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Alkyne-Modifier Serinol Phosphoramidite
Alkyne-Modifier Serinol Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1992-95 | 50µmoles | .038grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1992-90 | 100µmoles | .076grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1992-02 | 0.25grams | .25grams | 3.29 | 96.33 | 57.8 | 36.13 | 26.27 | 19.27 | 4.82 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1992-95 | 50µmoles | .038grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1992-90 | 100µmoles | .076grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1992-02 | 0.25grams | .25grams | 4.92 | .07 | 92 | 57.5 | 41.82 | 5.75 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1992-95 | 50µmoles | .038grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1992-90 | 100µmoles | .076grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1992-02 | 0.25grams | .25grams | 4.92 | .07 | 93.6 | 58.5 | 42.55 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
