Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Acridine Phosphoramidite
Acridine Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1973-02 | Acridine Phosphoramidite | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1973-90 | Acridine Phosphoramidite | 100µmoles | £236.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1973-95 | Acridine Phosphoramidite | 50µmoles | £132.00£125.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Acridine Phosphoramidite
Acridine Phosphoramidite
Glen Research
| Acridine Phosphoramidite |
Catalog Number: 10-1973-xx
Description: Acridine Phosphoramidite
| 1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite | ||
| Formula: C51H60N4O6PCl | M.W.: 891.53 | F.W.: 450.86 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling time is recommended.(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.) |
| Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.Note: NaOH is not compatible with dmf protecting groups. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
acridine LABELLING
Acridine phosphoramidite is designed to produce an oligonucleotidecontaining acridine at any position in the molecule. Acridine CPG is used tolabel the 3’-terminus. Acridine is an effective intercalating agent.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Acridine Phosphoramidite
Acridine Phosphoramidite
Glen Research
MSDS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Acridine Phosphoramidite
Acridine Phosphoramidite
Glen Research
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5"-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):Oligonucleotide Properties Calculator;http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
RESPONSE:Response:While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v)is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%.The impurity is not detected with AMA at RT for 2 hours.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Acridine Phosphoramidite
Acridine Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1973-02 | 0.25grams | .25grams | 2.8 | 80 | 48 | 30 | 21.82 | 16 | 4 |
| 10-1973-90 | 100µmoles | .089grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1973-95 | 50µmoles | .045grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1973-02 | 0.25grams | .25grams | 4.19 | .07 | 77.4 | 48.38 | 35.18 | 4.84 | |
| 10-1973-90 | 100µmoles | .089grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1973-95 | 50µmoles | .045grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1973-02 | 0.25grams | .25grams | 4.19 | .07 | 79 | 49.38 | 35.91 | ||
| 10-1973-90 | 100µmoles | .089grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1973-95 | 50µmoles | .045grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
