Oligo Synthesis : CEPs
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Abasic II Phosphoramidite
Abasic II Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1927-02 | Abasic II Phosphoramidite | 0.25g | £380.00£361.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1927-90 | Abasic II Phosphoramidite | 100µmoles | £120.00£114.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1927-95 | Abasic II Phosphoramidite | 50µmoles | £64.00£60.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Abasic II Phosphoramidite
Abasic II Phosphoramidite
Glen Research
| Abasic II Phosphoramidite |
Catalog Number: 10-1927-xx
Description: Abasic II Phosphoramidite
| 5-O-Dimethoxytrityl-1-O-tert-butyldimethylsilyl-2-deoxyribose-3-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H59N2O7PSi | M.W.: 750.98 | F.W.: 196.1 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 6 minute coupling time recommended. |
| Deprotection: Synthesize using acetyl-protected dC and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 min OR Synthesize using dmf-dG and deprotect in EtOH/30% Ammonium Hydroxide (1:3) for 24 hrs at room temperature.To generate the abasic site, dry the oligo down and take up in 0.25mL 80% Acetic acid.After 30 min at room temperature, add 0.25mL water and let sit for an additional 4 hrs.Quench with 0.5mL 2M TEAA and desalt on a Glen Gel-Pak™ column or equivalent.Store the oligo atpH 6-7 at 4°C or frozen.Do not dry; drying results in cleavage of the abasic site.See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
8-Amino-G is formed along with 8-oxo-G as themajor mutagenic lesions formed in DNA damage caused by 2-nitropropane.2-Nitropropane is an industrial solvent and a component of paints, dyes andvarnishes, and is also present in cigarette smoke. Thymine glycol(5,6-dihydroxy-5,6-dihydrothymine) is formed when thymine is subjected tooxidative stress, including ionizing radiation. Oxidation of the 5,6 double bondof Thymidine generates two chiral centers at C5 and C6. The cis-5R,6S form isgenerated as the predominant product along with the other diastereomer, thecis-5S,6R form. The presence of thymidine glycol in DNA has significantbiological consequences and many organisms possess specific repair enzymes forthe excision of this lesion. 2-Aminoimidazolone (Iz) and its hydrolysis productimidazolone (Z) are major oxidation products of G. Access to these two potentiallesions is not possible during oligonucleotide synthesis because they are sobase-labile. A suitable precursor, 8-methoxy-dG (8-OMe-dG), to dIz has now beendescribed. The conversion of 8-OMe-dG to dIz takes place by irradiation of theoligonucleotide (1 mM) in 50 mM sodium cacodylate buffer, pH 7, in the presenceof riboflavin (50 µM) for 2 minutes on a transilluminator (366 nm), underaerobic conditions at 4°C. Surprisingly for a photochemical reaction, theconversion is virtually quantitative.
Hydrolysis ofnucleoside residues in DNA occurs to generate abasic sites. Most commonly, dAsites are hydrolyzed causing depurination and leading to abasic residues. Forresearchers trying to determine if their source of depurination in chemicalsynthesis of DNA is reagent, fluidics or protocol-based, we offer adepurination-resistant dA monomer. A new chemical method allows the generationof abasic sites in double and single stranded oligonucleotides using very mildspecific conditions and with very low probability of side reactions. Theoriginal Abasic Phosphoramidite (10-1924) has been discontinued since itexhibits low coupling efficiency and the post-synthesis chemistry is fairlychallenging. Abasic II Phosphoramidite1 is the replacement for the preparationof a true abasic site. This product has the advantage of simplicity in that thesilyl group is removed post-synthesis using aqueous acetic acid. dSpacer hasalso been used successfully as a mimic of the highly base-labile abasic site.
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Abasic II Phosphoramidite
Abasic II Phosphoramidite
Glen Research
MSDS
Glen Report 21.1: TECHNICAL BRIEF - PREPARATION OF OLIGONUCLEOTIDES CONTAINING ABASIC SITES
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Abasic II Phosphoramidite
Abasic II Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1927-95 | 50µmoles | .038grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1927-90 | 100µmoles | .075grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1927-02 | 0.25grams | .25grams | 3.33 | 97.67 | 58.6 | 36.63 | 26.64 | 19.53 | 4.88 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1927-95 | 50µmoles | .038grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1927-90 | 100µmoles | .075grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1927-02 | 0.25grams | .25grams | 4.97 | .07 | 93 | 58.13 | 42.27 | 5.81 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1927-95 | 50µmoles | .038grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1927-90 | 100µmoles | .075grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1927-02 | 0.25grams | .25grams | 4.97 | .07 | 94.6 | 59.13 | 43 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
