Oligo Synthesis : CEPs
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7-deaza-8-aza-dA-CE Phosphoramidite
7-deaza-8-aza-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1083-02 | 7-deaza-8-aza-dA-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1083-90 | 7-deaza-8-aza-dA-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1083-95 | 7-deaza-8-aza-dA-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
7-deaza-8-aza-dA-CE Phosphoramidite
7-deaza-8-aza-dA-CE Phosphoramidite
Glen Research
| 7-deaza-8-aza-dA-CE Phosphoramidite |
Catalog Number: 10-1083-xx
Description: 7-deaza-8-aza-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N6-dimethylaminomethylidene-8-aza-7-deaza-2"-deoxyAdenosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H53N8O6P | M.W.: 808.91 | F.W.: 313.20 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
STRUCTURE/ACTIVITY RELATIONSHIP
The following products are used to investigate the effect on theactivity of an oligonucleotide when key structural elements are changed. The7-deaza purine monomers lack groups critical for hydrogen bonding.7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G andhave similar electron density. Their presence in oligos is slightly stabilizingrelative to A and G. Unlike G, PPG does not lead to aggregation and G-richoligos can be easily prepared and isolated. 5’-Fluorescein oligos with PPG atthe 5’-terminus are much less quenched than the equivalent G oligos. As a purineanalogue of Thymidine, 7-deaza-2’-deoxyXanthosine (7-deaza-dX) promises to haveinteresting effects on DNA structure of triplexes. 7-Deaza-dX also forms anon-standard base pair with a 2,4-diaminopyrimidine nucleoside analogue.Standard nucleobases have an unshared pair of electrons that project into theminor groove of duplex DNA. Enzymes that interact with DNA, polymerases, reversetranscriptases, restriction enzymes, etc., may use a hydrogen bond donatinggroup to contact the hydrogen bond acceptor in the minor groove.3-Deaza-2’-deoxyadenosine is very interesting in that it maintains the abilityfor regular Watson-Crick hydrogen bonding to T but is lacking the electron pairat the 3-position normally provided by N3.
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7-deaza-8-aza-dA-CE Phosphoramidite
7-deaza-8-aza-dA-CE Phosphoramidite
Glen Research
MSDS
Glen Report 15.1: MORE AND still MORE NOVEL MINOR BASES
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7-deaza-8-aza-dA-CE Phosphoramidite
7-deaza-8-aza-dA-CE Phosphoramidite
Glen Research
Frequently Asked Technical Question
QUESTION: Which should I use - psoralen C2 or psoralen C6?
RESPONSE:Psoralen C2 is designed to crosslink to a T residue adjacent to the 3"-terminus of the opposite strand of double stranded DNA.Psoralen C6 is intended to fulfill the same purpose but, with the longer spacer, crosslinks to the triple strand of triplex DNA.
Max excitation=330nm, observed at max 395nm
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7-deaza-8-aza-dA-CE Phosphoramidite
7-deaza-8-aza-dA-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 3.09 | 89.67 | 53.8 | 33.63 | 24.45 | 17.93 | 4.48 |
| 10-1083-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1083-95 | 50µmoles | .04grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 4.61 | .07 | 85.8 | 53.63 | 39 | 5.36 | |
| 10-1083-90 | 100µmoles | .081grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1083-95 | 50µmoles | .04grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 4.61 | .07 | 87.4 | 54.63 | 39.73 | ||
| 10-1083-90 | 100µmoles | .081grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1083-95 | 50µmoles | .04grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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