Oligo Synthesis : CEPs
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6-Thio-G-CE Phosphoramidite
6-Thio-G-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3072-02 | 6-Thio-G-CE Phosphoramidite | 0.25g | £960.00£912.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3072-90 | 6-Thio-G-CE Phosphoramidite | 100µmoles | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3072-95 | 6-Thio-G-CE Phosphoramidite | 50µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
6-Thio-G-CE Phosphoramidite
6-Thio-G-CE Phosphoramidite
Glen Research
| 6-Thio-G-CE Phosphoramidite |
Catalog Number: 10-3072-xx
Description: 6-Thio-G-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine,2"-O-TBDMS-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C53H71N8O8PSSi | M.W.: 1039.31 | F.W.: 361.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015). |
| Deprotaction: Treat column with 1M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection.See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf). Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 2-3 days minor rna phosphoramidites (TBDMS PROTECTED)Inosine and 5-Methyl-Uridine are useful for analyzing RNAstructure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine havebeen used for crystallography studies and cross-linking experiments.6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, aswell as in RNA-protein interactions. The removal of the silyl protecting groupwithout interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride(TBAF) leads to degradation of the thio-nucleotide analogue and should not beused. 2-Aminopurine riboside is useful for analyzing RNA structure and activityrelationships, for example, in ribozyme studies. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
6-Thio-G-CE Phosphoramidite
6-Thio-G-CE Phosphoramidite
Glen Research
MSDS
| Glen Report 17.1: Products for siRNA Research |
| Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
6-Thio-G-CE Phosphoramidite
6-Thio-G-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 2.41 | 67 | 40.2 | 25.13 | 18.27 | 13.4 | 3.35 |
| 10-3072-90 | 100µmoles | .104grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3072-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 3.59 | .07 | N/A | N/A | 13.63 | N/A | |
| 10-3072-90 | 100µmoles | .104grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
| 10-3072-95 | 50µmoles | .052grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 3.59 | .07 | N/A | 10.81 | 10.15 | ||
| 10-3072-90 | 100µmoles | .104grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
| 10-3072-95 | 50µmoles | .052grams | .75 | .07 | N/A | 1.65 | 1.55 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
