Oligo Synthesis : CEPs
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5-Me-dC-CE Phosphoramidite
5-Me-dC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1060-02 | 5-Me-dC-CE Phosphoramidite | 0.25g | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1060-90 | 5-Me-dC-CE Phosphoramidite | 100µmoles | £50.00£47.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5-Me-dC-CE Phosphoramidite
5-Me-dC-CE Phosphoramidite
Glen Research
| 5-Me-dC-CE Phosphoramidite |
Catalog Number: 10-1060-xx
Description: 5-Me-dC-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-5-methyl-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C47H54N5O8P | M.W.: 847.9 | F.W.: 303.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
bases affecting duplex stability
C-5 methyl pyrimidine nucleosides are known to stabilize duplexesrelative to the non-methylated bases. Therefore, enhanced binding can beachieved using 5-methyl-dC in place of dC, duplex melting temperature beingincreased by 1.3°. Improved stacking in this case is believed to be broughtabout by elimination of water molecules from the duplex. 2,6-Diaminopurine2"-deoxyriboside (2-amino-dA) forms an additional hydrogen bond with Thymidine,thereby leading to duplex stabilization with a melting temperature increase of3°. Our 2-amino-dA monomer exhibits fast and effective deprotection in ammoniumhydroxide and it is stabilized to depurination during synthesis. Sequences withhigh GC content may contain mismatches and still hybridize because of the highstability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC)hybridizes specifically to natural dG but the stability of the base pair isreduced to about the level of an AT base pair.AP-dC (G-clamp) enhancesoligo hybridization since the AP-C....G base pair contains 4 hydrogen bonds,which makes the interaction much stronger than the regular C....G base pair withits 3 hydrogen bonds.
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5-Me-dC-CE Phosphoramidite
5-Me-dC-CE Phosphoramidite
Glen Research
MSDS
Glen Report 11.1: USING MODIFIED BASES TO OPTIMIZE HYBRIDIZATION
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC-CE Phosphoramidite
5-Me-dC-CE Phosphoramidite
Glen Research
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC-CE Phosphoramidite
5-Me-dC-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1060 | 5-Me-dC | 277 | 9 | 5.7 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1060-02 | 0.25grams | .25grams | 2.95 | 85 | 51 | 31.88 | 23.18 | 17 | 4.25 |
| 10-1060-90 | 100µmoles | .085grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1060-02 | 0.25grams | .25grams | 4.4 | .07 | 81.6 | 51 | 37.09 | 5.1 | |
| 10-1060-90 | 100µmoles | .085grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1060-02 | 0.25grams | .25grams | 4.4 | .07 | 83.2 | 52 | 37.82 | ||
| 10-1060-90 | 100µmoles | .085grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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