Athens Research/Cambio - Excellence in Molecular Biology/0.25g/10-1018-02
商品编号：
10-1018-02
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1018-02 | 5-Me-dC Brancher Phosphoramidite | 0.25g | £404.00£383.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1018-90 | 5-Me-dC Brancher Phosphoramidite | 100µmoles | £164.00£155.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
| 5-Me-dC Brancher Phosphoramidite |
Catalog Number: 10-1018-xx
Description: 5-Me-dC Brancher Phosphoramidite
| 5"-Dimethoxytrityl-N4-(O-levulinyl-6-oxyhexyl)-5-Methyl-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H68N5O10P | M.W.: 942.10 | F.W.: 402.36 |
Diluent: Anhydrous Acetonitrile |
| Coupling: dC Brancher reacts in a manner identical to normal phosphoramidites. |
| Levulinyl Deprotection:The levulinyl protecting group can be selectively removed without cleavage of the oligonucleotide from the CPG by treatment with 0.5 M Hydrazine hydrate in 1:1 pyridine/acetic acid.[Note hydrazine hydrate is a violent poison that is both volatile and readily absorbed through skin]Fit the column with syringes and push the solution back and forth across the column.Let sit for 15 minutes at room temperature.Rinse the column with 1.5 mL of 1:1 pyridine/acetic acid (3x) and then 1.5 mL of ACN (3x).Dry CPG under a stream of argon and proceed with the synthesis of the branching sequence.If a non-branching control is desired, simply deprotect in ammonium hydroxide as required by the nucleobases.Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
BRANCHING PHOSPHORAMIDITE
A branching monomer is required to construct comb-likeoligonucleotide probes. The developers of the comb system from ChironCorporation evaluated3 several protecting groups for the branch pointand chose levulinyl (LEV), which is specifically removed using a reagentcontaining hydrazine hydrate, acetic acid and pyridine.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
MSDS
| Glen Report 18.1: Synthesis of Branched DNA with a Comb Structure |
| Glen Report 24.1: Technical Brief - Compatibility of UltraMild Monomers or Ac-dC with Hydrazine Reagent |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| 10-1018-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 3.96 | .07 | 72.8 | 45.5 | 33.09 | 4.55 | |
| 10-1018-90 | 100µmoles | .094grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 3.96 | .07 | 74.4 | 46.5 | 33.82 | ||
| 10-1018-90 | 100µmoles | .094grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品
Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。
品牌分类
转铁蛋白和apotranferrins
Lab Reagents: Smart Buffers and Reagents
分子生物学用酶：分子生物学用酶
蛋白质纯化：阿波罗20毫升超滤
免疫球蛋白
qPCR：基因分析和基因面板
PCR : PCR Products
牛奶和血小板蛋白
DNA & RNA Sequencing : Dye Terminator clean-up
Aptamers: Catalogue Aptamers
Peptide Synthesis : Amino Acids
运输和其他蛋白质
DNA: Whole DNA
STEM学习：STEM工具包
低内毒素水平蛋白质
提取和纯化-RNA：组织/培养
转染-EZ生物系统：细胞系
凝血因子
分析试剂盒
抗体
Gel Electrophoresis: Gel Electrophoresis
中性粒细胞蛋白
Transfection - Biontex: Proteofection
体外毒理学：致突变性试剂盒
寡糖合成：CEPs
动物蛋白
Extraction & Purification - DNA: Plasmid
人类蛋白质
染色体绘制：人类
脂蛋白和载脂蛋白
样本拭子：鼻咽拭子
酶和抑制剂
细胞培养污染控制：检测
Antibodies: Collagenase
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