Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
5-I-dC-CE Phosphoramidite
5-I-dC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1081-02 | 5-I-dC-CE Phosphoramidite | 0.25g | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1081-90 | 5-I-dC-CE Phosphoramidite | 100µmoles | £108.00£102.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5-I-dC-CE Phosphoramidite
5-I-dC-CE Phosphoramidite
Glen Research
| 5-I-dC-CE Phosphoramidite |
Catalog Number: 10-1081-xx
Description: 5-I-dC-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-5-iodo-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C46H51IN5O8P | M.W.: 959.83 | F.W.: 415.08 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Mild deprotection: Ammonium Hyrdoxide for 24 hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
HALOGENATED NUCLEOSIDES
Brominated and iodinated nucleosides are used in crystallographystudies of oligonucleotide structure. They are also photolabile and are used forcross-linking studies to probe the structure of protein-DNA complexes.Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used asprobes.
HALOGENATED NUCLEOSIDES
Brominated and iodinated nucleosides are used in crystallographystudies of oligonucleotide structure. They are also photolabile and are used forcross-linking studies to probe the structure of protein-DNA complexes.Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used asprobes.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-I-dC-CE Phosphoramidite
5-I-dC-CE Phosphoramidite
Glen Research
MSDS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-I-dC-CE Phosphoramidite
5-I-dC-CE Phosphoramidite
Glen Research
Oligonucleotides containinga bromo or iodo group are prepared conventionally with the exception that deprotection is carried out in ammonium hydroxide at room temperature for 24 hours. Under these conditions, degradation of the halogen group was less than 2%.
Frequently Asked Technical Question
QUESTION: What are the extinction coefficients for propynyl-dC and dU?Also Halogenated dC and dU derivatives?Others?
RESPONSE:See: Table of Extinction Coefficients
REFERENCE(S):B. Froehler, GileadM. PowellR. Somers
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-I-dC-CE Phosphoramidite
5-I-dC-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1081 | 5-I-dC | 293 | 5.7 | 3.3 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1081-02 | 0.25grams | .25grams | 2.6 | 73.33 | 44 | 27.5 | 20 | 14.67 | 3.67 |
| 10-1081-90 | 100µmoles | .096grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1081-02 | 0.25grams | .25grams | 3.89 | .07 | 71.4 | 44.63 | 32.45 | 4.46 | |
| 10-1081-90 | 100µmoles | .096grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1081-02 | 0.25grams | .25grams | 3.89 | .07 | 73 | 45.63 | 33.18 | ||
| 10-1081-90 | 100µmoles | .096grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
