Oligo Synthesis : CEPs
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5"-Hexynyl Phosphoramidite
5"-Hexynyl Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1908-02 | 5"-Hexynyl Phosphoramidite | 0.25g | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1908-90 | 5"-Hexynyl Phosphoramidite | 100µmoles | £48.00£45.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Hexynyl Phosphoramidite
5"-Hexynyl Phosphoramidite
Glen Research
| 5"-Hexynyl Phosphoramidite |
Catalog Number: 10-1908-xx
Description: 5"-Hexynyl Phosphoramidite
| 6-Hexyn-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C15H27N2O2P | M.W.: 298.36 | F.W.: 160.11 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable tostandard deprotection conditions and exhibit a slightly increased retention timeon RP HPLC. Azides are not compatible with oligonucleotide synthesis usingphosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHSEster is used1 for azido-modification of amines at either the 3’-endor the 5’-end of an oligo and it can even be used for internal modification onan Amino-Modifier-C6 dX residue within the sequence. Specific to the5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. Thismodifier can then be easily transformed into a 5’-azido group by displacement ofbromide using sodium azide.2 Alkyne NHS ester allows thefunctionalization of an amino moiety in a variety of molecules, including DNAand RNA oligonucleotides as well as peptides or proteins. We also offer asynthesis support for labelling the 3’ terminus of oligonucleotides with analkyne group for use in Click Chemistry. This builds upon our 1,3-diol productportfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide(prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding5’-azide.
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5"-Hexynyl Phosphoramidite
5"-Hexynyl Phosphoramidite
Glen Research
MSDS
Glen Report 19.1
Glen Report 20.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Hexynyl Phosphoramidite
5"-Hexynyl Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1908-90 | 100µmoles | .03grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1908-02 | 0.25grams | .25grams | 8.38 | 266 | 159.6 | 99.75 | 72.55 | 53.2 | 13.3 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1908-90 | 100µmoles | .03grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1908-02 | 0.25grams | .25grams | 12.51 | .07 | 243.8 | 152.38 | 110.82 | 15.24 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1908-90 | 100µmoles | .03grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1908-02 | 0.25grams | .25grams | 12.51 | .07 | 245.4 | 153.38 | 111.55 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
