Oligo Synthesis : CEPs
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5"-Dabsyl-dT-CE Phosphoramidite
5"-Dabsyl-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1532-02 | 5"-Dabsyl-dT-CE Phosphoramidite | 0.25g | £620.00£589.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1532-90 | 5"-Dabsyl-dT-CE Phosphoramidite | 100µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Dabsyl-dT-CE Phosphoramidite
5"-Dabsyl-dT-CE Phosphoramidite
Glen Research
| 5"-Dabsyl-dT-CE Phosphoramidite |
Catalog Number: 10-1532-xx
Description: 5"-Dabsyl-dT-CE Phosphoramidite
| 5"-Dabsyl-2"-deoxyThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C33H44N7O8PS | M.W.: 729.78 | F.W.: 591.53 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dGwith acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2days |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescencequenched in solution unless it is hybridized to the target sequence.Consequently, the design of a molecular beacon requires a fluorophore to be inone part of the sequence and the quencher molecule to be in another, with bothmolecules being separated from the oligonucleotide by a hydrocarbon spacer. TheDabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and3’-Dabcyl CPG are used to prepare probes with the quencher blocking the3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminusand Dabcyl-dT places it within the sequence, leaving the 3’-terminus availablefor polymerase extension.
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5"-Dabsyl-dT-CE Phosphoramidite
5"-Dabsyl-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 19.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Dabsyl-dT-CE Phosphoramidite
5"-Dabsyl-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1532-02 | 0.25grams | .25grams | 3.43 | 101 | 60.6 | 37.88 | 27.55 | 20.2 | 5.05 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1532-02 | 0.25grams | .25grams | 5.11 | .07 | 95.8 | 59.88 | 43.55 | 5.99 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1532-02 | 0.25grams | .25grams | 5.11 | .07 | 97.4 | 60.88 | 44.27 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
