Oligo Synthesis : CEPs
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5"-Dabcyl Phosphoramidite
5"-Dabcyl Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-5912-02 | 5"-Dabcyl Phosphoramidite | 0.25g | £520.00£494.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5912-90 | 5"-Dabcyl Phosphoramidite | 100µmoles | £180.00£171.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5912-95 | 5"-Dabcyl Phosphoramidite | 50µmoles | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Dabcyl Phosphoramidite
5"-Dabcyl Phosphoramidite
Glen Research
| 5"-Dabcyl Phosphoramidite |
Catalog Number: 10-5912-xx
Description: 5"-Dabcyl Phosphoramidite
| 6-(N-4"-carboxy-4-(dimethylamino)azobenzene)-aminohexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C30H45N6O3P | M.W.: 568.69 | F.W.: 430.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended |
| Deprotection:No changes needed from standard methodrecommended by synthesizermanufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescencequenched in solution unless it is hybridized to the target sequence.Consequently, the design of a molecular beacon requires a fluorophore to be inone part of the sequence and the quencher molecule to be in another, with bothmolecules being separated from the oligonucleotide by a hydrocarbon spacer. TheDabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and3’-Dabcyl CPG are used to prepare probes with the quencher blocking the3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminusand Dabcyl-dT places it within the sequence, leaving the 3’-terminus availablefor polymerase extension.
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5"-Dabcyl Phosphoramidite
5"-Dabcyl Phosphoramidite
Glen Research
MSDS
Glen Report 12.1
Glen Report 21.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Dabcyl Phosphoramidite
5"-Dabcyl Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-5912 | 468nm | 32.0 (L/mmole-cm) | 11.1 (L/mmole-cm) |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5912-02 | 0.25grams | .25grams | 4.4 | 133.33 | 80 | 50 | 36.36 | 26.67 | 6.67 |
| 10-5912-90 | 100µmoles | .057grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5912-95 | 50µmoles | .028grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5912-02 | 0.25grams | .25grams | 6.56 | .07 | 124.8 | 78 | 56.73 | 7.8 | |
| 10-5912-90 | 100µmoles | .057grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5912-95 | 50µmoles | .028grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5912-02 | 0.25grams | .25grams | 6.56 | .07 | 126.4 | 79 | 57.45 | ||
| 10-5912-90 | 100µmoles | .057grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5912-95 | 50µmoles | .028grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
