Oligo Synthesis : CEPs
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5"-Amino-Modifier C12
5"-Amino-Modifier C12
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1912-02 | 5"-Amino-Modifier C12 | 0.25g | £240.00£228.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1912-90 | 5"-Amino-Modifier C12 | 100µmoles | £72.00£68.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Amino-Modifier C12
5"-Amino-Modifier C12
Glen Research
| 5"-Amino-Modifier C12 |
TERMINUS MODIFIERS
Glen Research 5’-Modifiers are designed for use in DNA synthesizers to functionalize the 5’-terminus of the target oligonucleotide. The 5’-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.
The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT , which is more stable than MMT , does not reattach itself to an amine. We now offer 5’-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.
5’-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.
Catalog Number: 10-1912-xx
Description: 5"-Amino-Modifier C12
| 12-(4-Monomethoxytritylamino)dodecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H60N3O3P | M.W.: 673.92 | F.W.: 263.32 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect as required by nucleobases.WARNING: Drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group.Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
TERMINUS MODIFIERS
Glen Research 5’-Modifiers are designed for use in DNA synthesizers to functionalize the 5’-terminus of the target oligonucleotide. The 5’-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.
The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT , which is more stable than MMT , does not reattach itself to an amine. We now offer 5’-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.
5’-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Amino-Modifier C12
5"-Amino-Modifier C12
Glen Research
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Amino-Modifier C12
5"-Amino-Modifier C12
Glen Research
5"-Amino-Modifier C12
| 12-(4-Monomethoxytritylamino)dodecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H60N3O3P | M.W.: 673.92 | F.W.: 263.32 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect as required by nucleobases.WARNING: Drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Material Safety Data Sheet | Technical Notes |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 3.71 | 110.33 | 66.2 | 41.38 | 30.09 | 22.07 | 5.52 |
| 10-1912-90 | 100µmoles | .067grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 5.54 | .067 | 104.4 | 65.25 | 47.45 | 6.53 | |
| 10-1912-90 | 100µmoles | .067grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 5.54 | .067 | 106 | 66.25 | 48.18 | ||
| 10-1912-90 | 100µmoles | .067grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Amino-Modifier C12
5"-Amino-Modifier C12
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 3.71 | 110.33 | 66.2 | 41.38 | 30.09 | 22.07 | 5.52 |
| 10-1912-90 | 100µmoles | .067grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 5.54 | .07 | 104.4 | 65.25 | 47.45 | 6.53 | |
| 10-1912-90 | 100µmoles | .067grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1912-02 | 0.25grams | .25grams | 5.54 | .07 | 106 | 66.25 | 48.18 | ||
| 10-1912-90 | 100µmoles | .067grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
