Oligo Synthesis : CEPs
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5"-Amino-dT-CE Phosphoramidite
5"-Amino-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1932-02 | 5"-Amino-dT-CE Phosphoramidite | 0.25g | £320.00£304.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1932-90 | 5"-Amino-dT-CE Phosphoramidite | 100µmoles | £120.00£114.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Amino-dT-CE Phosphoramidite
5"-Amino-dT-CE Phosphoramidite
Glen Research
| 5"-Amino-dT-CE Phosphoramidite |
Catalog Number: 10-1932-xx
Description: 5"-Amino-dT-CE Phosphoramidite
| 5"-monomethoxytritylamino-2"-deoxyThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H48N5O6P | M.W.: 713.81 | F.W.: 303.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect as required by nucleobases.WARNING: As with other trityl-protected amines, drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. See Technical Bulletin for details (Technical Bulletin). |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
CHAIN TERMINATORS
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.15’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
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5"-Amino-dT-CE Phosphoramidite
5"-Amino-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1
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5"-Amino-dT-CE Phosphoramidite
5"-Amino-dT-CE Phosphoramidite
Glen Research
Technical Notes
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5"-Amino-dT-CE Phosphoramidite
5"-Amino-dT-CE Phosphoramidite
Glen Research
5"-Amino-dT terminates an oligonucleotide with a 5"-amino group which may be used for attaching a peptide of PNA sequence.
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1932-02 | 0.25grams | .25grams | 3.5 | 103.33 | 62 | 38.75 | 28.18 | 20.67 | 5.17 |
| 10-1932-90 | 100µmoles | .071grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1932-02 | 0.25grams | .25grams | 5.23 | .07 | 98.2 | 61.38 | 44.64 | 6.14 | |
| 10-1932-90 | 100µmoles | .071grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1932-02 | 0.25grams | .25grams | 5.23 | .07 | 99.8 | 62.38 | 45.36 | ||
| 10-1932-90 | 100µmoles | .071grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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