Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
5’- DBCO-TEG Phosphoramidite
5’- DBCO-TEG Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1941-02 | 5’-DBCO-TEG Phosphoramidite | 0.25g | £620.00£589.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1941-90 | 5’-DBCO-TEG Phosphoramidite | 100 µmole | £184.00£174.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1941-95 | 5’-DBCO-TEG Phosphoramidite | 50 µmole | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5’- DBCO-TEG Phosphoramidite
5’- DBCO-TEG Phosphoramidite
Glen Research
5Õ-DBCO-TEG Phosphoramidite
Catalog Number: 10-1941-xx
Description: 5Õ-DBCO-TEG Phosphoramidite
| 10-(6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-capramido-N-ethyl)-O-triethyleneglycol-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C38H53N4O7P | M.W.: 708.82 | F.W.: 570.57 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 10 minute coupling time recommended. |
| Deprotection: Compatible with 30% ammonium hydroxide for 2 hours at 65 °C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results. |
| Storage: Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
COPPER-FREE CLICK CHEMISTRY
At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
- Simple to use
- Stable in solution on the synthesizer
- Stable to ammonium hydroxide and AMA
- Excellent click performance in 17 hours or less at room temperature
From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5’-DBCO-TEG Phosphoramidite for preparing oligos with a 5’-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5’- DBCO-TEG Phosphoramidite
5’- DBCO-TEG Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 24.1: New Product - Dibenzocyclooctyl (DBCO) Copper-Free Click Chemistry |
| Glen Report 24.2: New Product - DBCO-dT for Copper-Free Click Chemistry |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5’- DBCO-TEG Phosphoramidite
5’- DBCO-TEG Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1941-95 | 50µmoles | .035grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1941-90 | 100µmoles | .071grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1941-02 | 0.25grams | .25grams | 3.53 | 104.33 | 62.6 | 39.13 | 28.45 | 20.87 | 5.22 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1941-95 | 50µmoles | .035grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1941-90 | 100µmoles | .071grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1941-02 | 0.25grams | .25grams | 5.26 | .07 | 98.8 | 61.75 | 44.91 | 6.18 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1941-95 | 50µmoles | .035grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1941-90 | 100µmoles | .071grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1941-02 | 0.25grams | .25grams | 5.26 | .07 | 100.4 | 62.75 | 45.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
