Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
5,6-Dihydro-dU-CE Phosphoramidite
5,6-Dihydro-dU-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1550-02 | 5,6-Dihydro-dU-CE Phosphoramidite | 0.25g | £476.00£452.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1550-90 | 5,6-Dihydro-dU-CE Phosphoramidite | 100µmoles | £156.00£148.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5,6-Dihydro-dU-CE Phosphoramidite
5,6-Dihydro-dU-CE Phosphoramidite
Glen Research
| 5,6-Dihydro-dU-CE Phosphoramidite |
Catalog Number: 10-1550-xx
Description: 5,6-Dihydro-dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-5,6-dihydro-2"-deoxyUridine),3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H49N4O8P | M.W.: 732.81 | F.W.: 292.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA:10-1601-xx,dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature or 2 hours at room temperature in Ammonium Hydroxide. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
DNA DAMAGE/REPAIR
Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5,6-Dihydro-dU-CE Phosphoramidite
5,6-Dihydro-dU-CE Phosphoramidite
Glen Research
MSDS
Glen Report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5,6-Dihydro-dU-CE Phosphoramidite
5,6-Dihydro-dU-CE Phosphoramidite
Glen Research
Oligonucleotides synthesised using 5,6-dihydro-dU or 5,6-dihydro-dT and UltraMILD monomers can be cleaved using either concentrated ammonium hydroxide or 50mM potassium carbonate in anhydrous methanol. Complete cleavage and deprotection can be accomplished at room temperature in 2-4 hours without damaging either the dihydro-dU or dihydro-dT bases.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5,6-Dihydro-dU-CE Phosphoramidite
5,6-Dihydro-dU-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1550 | dihydro-dU | 210 | 6.3 | <0.1 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1550-02 | 0.25grams | .25grams | 3.41 | 100.33 | 60.2 | 37.63 | 27.36 | 20.07 | 5.02 |
| 10-1550-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1550-02 | 0.25grams | .25grams | 5.09 | .07 | 95.4 | 59.63 | 43.36 | 5.96 | |
| 10-1550-90 | 100µmoles | .073grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1550-02 | 0.25grams | .25grams | 5.09 | .07 | 97 | 60.63 | 44.09 | ||
| 10-1550-90 | 100µmoles | .073grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
