Oligo Synthesis : CEPs
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5,6-Dihydro-dT-CE Phosphoramidite
5,6-Dihydro-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1530-02 | 5,6-Dihydro-dT-CE Phosphoramidite | 0.25g | £476.00£452.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1530-90 | 5,6-Dihydro-dT-CE Phosphoramidite | 100µmoles | £156.00£148.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5,6-Dihydro-dT-CE Phosphoramidite
5,6-Dihydro-dT-CE Phosphoramidite
Glen Research
| 5,6-Dihydro-dT-CE Phosphoramidite |
Catalog Number: 10-1530-xx
Description: 5,6-Dihydro-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-5,6-dihydro-2"-deoxyThymidine),3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H51N4O8P | M.W.: 746.84 | F.W.: 306.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: 2 hours at room temperature in K2CO3/MeOH or 2 hours at room temperature in NH4OH.ÒUltraMILDÓ monomers must be used. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
DNA Damage/Repair
Cellular DNA is constantly being damaged by oxidation andalkylation, by free radicals, and by ultraviolet and ionizing radiation. Thebody has therefore evolved a number of repair enzyme systems to excise andrepair these lesions. The 8-oxo purine monomers allow investigation of thestructure and activity of oligonucleotides containing an 8-oxo mutation which isformed naturally when DNA is subjected to oxidative conditions or ionizingradiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that arestructural components of alanine transfer RNA. Dihydrouracil and the hydroxypyrimidines are major base damage products formed by exposure of DNA to ionizingradiation.
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5,6-Dihydro-dT-CE Phosphoramidite
5,6-Dihydro-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1: MORE NOVEL MONOMERS
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5,6-Dihydro-dT-CE Phosphoramidite
5,6-Dihydro-dT-CE Phosphoramidite
Glen Research
Oligonucleotides synthesised using 5,6-dihydro-dU or 5,6-dihydro-dT and UltraMILD monomers can be cleaved using either concentrated ammonium hydroxide or 50mM potassium carbonate in anhydrous methanol. Complete cleavage and deprotection can be accomplished at room temperature in 2-4 hours without damaging either the dihydro-dU or dihydro-dT bases.
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5,6-Dihydro-dT-CE Phosphoramidite
5,6-Dihydro-dT-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1530 | dihydro-dT | 210 | 6.3 | <0.1 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1530-02 | 0.25grams | .25grams | 3.35 | 98.33 | 59 | 36.88 | 26.82 | 19.67 | 4.92 |
| 10-1530-90 | 100µmoles | .075grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1530-02 | 0.25grams | .25grams | 5 | .07 | 93.6 | 58.5 | 42.55 | 5.85 | |
| 10-1530-90 | 100µmoles | .075grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1530-02 | 0.25grams | .25grams | 5 | .07 | 95.2 | 59.5 | 43.27 | ||
| 10-1530-90 | 100µmoles | .075grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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