Oligo Synthesis : CEPs
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4-Thio-dU-CE Phosphoramidite
4-Thio-dU-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1052-02 | 4-Thio-dU-CE Phosphoramidite | 0.25g | £700.00£665.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1052-90 | 4-Thio-dU-CE Phosphoramidite | 100µmoles | £240.00£228.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1052-95 | 4-Thio-dU-CE Phosphoramidite | 50µmoles | £132.00£125.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
4-Thio-dU-CE Phosphoramidite
4-Thio-dU-CE Phosphoramidite
Glen Research
| 4-Thio-dU-CE Phosphoramidite |
Catalog Number: 10-1052-xx
Description: 4-Thio-dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxy-4-(2-cyanoethylthio)-Uridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H50N5O7PS | M.W.: 799.93 | F.W.: 306.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
Demand for sulfur modified bases continues to expand for investigations of oligonucleotide structure, but primarily for cross-linking purposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications for photo-crosslinking and photoaffinity labelling experiments. Oligos containing 2-thio-dT are useful in examining protein-DNA interaction by acting as photosensitizing probes. The thiocarbonyl group in 2-thio-dT is especially interesting in that it is available to react with compounds associating with the minor groove of DNA. 2-Amino-A forms a very stable base pair with T containing three hydrogen bonds but the stability of the base pair with 2-thio-T is greatly diminished. Due to steric interactions between the 2-thio group of thymidine and the 2-amino group of 2-amino-A, the base pair contains only a single hydrogen bond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity for natural oligonucleotides but show little affinity for other similar oligos even of a complementary sequence.
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4-Thio-dU-CE Phosphoramidite
4-Thio-dU-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1: MORE NOVEL MONOMERS
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4-Thio-dU-CE Phosphoramidite
4-Thio-dU-CE Phosphoramidite
Glen Research
6-Thio-dG, 4-Thio-dT and 4-Thio-dU are protected as the S-cyanoethyl ether which is stable during synthesis and readily removed by ammonium hydroxide. It is critical to add 50mM sodium hydrosulfide (NaSH) to the ammonium hydroxide used for deprotection. Especially if room temperature deprotection is carried out, this technique radically reduces the level of ammonolysis which would lead to undesired aminated bases. Moreover, it is also desirable to remove the cyanoethyl protecting group (1M DBU in acetonitrile, 2-5h/RT) prior to the ammonium hydroxide cleavage and deprotection.
4-Thio-dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxy-4-(2-cyanoethylthio)-Uridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H50N5O7PS | M.W.: 799.93 | F.W.: 306.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1052 | 4-thio-dU | 330 | 30.4 | 3.6 |
Literature Highlights
| Glen Report 10.1: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 3.13 | 91 | 54.6 | 34.13 | 24.82 | 18.2 | 4.55 |
| 10-1052-90 | 100µmoles | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1052-95 | 50µmoles | .04grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .067 | 86.8 | 54.25 | 39.45 | 5.43 | |
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1052-95 | 50µmoles | .04grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .067 | 88.4 | 55.25 | 40.18 | ||
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1052-95 | 50µmoles | .04grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
4-Thio-dU-CE Phosphoramidite
4-Thio-dU-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1052 | 4-thio-dU | 330 | 30.4 | 3.6 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 3.13 | 91 | 54.6 | 34.13 | 24.82 | 18.2 | 4.55 |
| 10-1052-90 | 100µmoles | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1052-95 | 50µmoles | .04grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .07 | 86.8 | 54.25 | 39.45 | 5.43 | |
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1052-95 | 50µmoles | .04grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .07 | 88.4 | 55.25 | 40.18 | ||
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1052-95 | 50µmoles | .04grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
