Oligo Synthesis : CEPs
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4-Thio-dT-CE Phosphoramidite
4-Thio-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1034-02 | 4-Thio-dT-CE Phosphoramidite | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1034-90 | 4-Thio-dT-CE Phosphoramidite | 100µmoles | £236.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1034-95 | 4-Thio-dT-CE Phosphoramidite | 50µmoles | £132.00£125.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
4-Thio-dT-CE Phosphoramidite
4-Thio-dT-CE Phosphoramidite
Glen Research
| 4-Thio-dT-CE Phosphoramidite |
Catalog Number: 10-1034-xx
Description: 4-Thio-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxy-4-(2-cyanoethylthio)-Thymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H52N5O7PS | M.W.: 813.95 | F.W.: 320.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time.Use 0.02 M Iodine for Oxidation. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
The C-nucleoside2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets.2-Aminopurine lacks groups critical for hydrogen bonding and is a mildlyfluorescent base.
Demand for sulfur modified bases continues to expand forinvestigations of oligonucleotide structure, but primarily for cross-linkingpurposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications forphoto cross-linking and photoaffinity labelling experiments. Oligos containing2-thio-dT are useful in examining protein-DNA interaction by acting asphotosensitizing probes. The thiocarbonyl group in 2-thio-dT is especiallyinteresting in that it is available to react with compounds associating with theminor groove of DNA. 2-Amino-A forms a very stable base pair with T containingthree hydrogen bonds but the stability of the base pair with 2-thio-T is greatlydiminished. Due to steric interactions between the 2-thio group of thymidine andthe 2-amino group of 2-amino-A, the base pair contains only a single hydrogenbond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity fornatural oligonucleotides but show little affinity for other similar oligos evenof a complementary sequence.
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4-Thio-dT-CE Phosphoramidite
4-Thio-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 8.2: TRANSCRIPTION TERMINATOR, 2-THIO- AND 4-THIO-THYMIDINE
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4-Thio-dT-CE Phosphoramidite
4-Thio-dT-CE Phosphoramidite
Glen Research
6-Thio-dG, 4-Thio-dT and 4-Thio-dU are protected as the S-cyanoethyl ether which is stable during synthesis and readily removed by ammonium hydroxide. It is critical to add 50mM sodium hydrosulfide (NaSH) to the ammonium hydroxide used for deprotection. Especially if room temperature deprotection is carried out, this technique radically reduces the level of ammonolysis which would lead to undesired aminated bases. Moreover, it is also desirable to remove the cyanoethyl protecting group (1M DBU in acetonitrile, 2-5h/RT) prior to the ammonium hydroxide cleavage and deprotection.
Frequently Asked Technical Question
QUESTION: Can 4-thiodU be used for crosslinking and how is it prepared?
RESPONSE:4-thioU is efficiently activated for crosslinking by exposure to long-wavelength UV light for up to 10 minutes.Crosslinks are formed with RNA and proteins.
Oligonucleotides containing 4-thiodU (10-1051) and 4-thioT (10-1033) can be produced from the triazole modified phosphoramidites using the convertible nucleoside strategy (1,2).However, we now offer the nuclesides 4-thio-dT (10-1034), 4-thio-dU (10-1052), 2-thio-dT (10-1036), and 4-thio-U (10-3052) for direct incorporation into oligonucleotides and the convertible monomers have been discontinued.A further enhancement of this strategy used 4-thioxU as an intermediated in the preparation of thiocarbonyl crosslinkers (3).
FERENCE(S):(1)Y.Z. Xu,Q. Zheng, and P.F. Swann, J. Org. Chem., 1992, 57, 3841.(2)The Glen Report, 1993, 6.1, 1, and references cited therein.(3) R.S. Coleman and J.M. Siedlecki, Journal of the American Chemical Society, 1992, 114, 9229-9230.
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4-Thio-dT-CE Phosphoramidite
4-Thio-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1034-02 | 0.25grams | .25grams | 3.07 | 89 | 53.4 | 33.38 | 24.27 | 17.8 | 4.45 |
| 10-1034-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1034-95 | 50µmoles | .041grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1034-02 | 0.25grams | .25grams | 4.58 | .07 | 85.2 | 53.25 | 38.73 | 5.33 | |
| 10-1034-90 | 100µmoles | .081grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1034-95 | 50µmoles | .041grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1034-02 | 0.25grams | .25grams | 4.58 | .07 | 86.8 | 54.25 | 39.45 | ||
| 10-1034-90 | 100µmoles | .081grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1034-95 | 50µmoles | .041grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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