Oligo Synthesis : CEPs
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3"-dT-CE Phosphoramidite
3"-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1084-02 | 3"-dT-CE Phosphoramidite | 0.25g | £1,260.00£1,140.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1084-90 | 3"-dT-CE Phosphoramidite | 100µmoles | £392.00£342.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1084-95 | 3"-dT-CE Phosphoramidite | 50µmoles | £204.00£171.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
3"-dT-CE Phosphoramidite
3"-dT-CE Phosphoramidite
Glen Research
| 3"-dT-CE Phosphoramidite |
Catalog Number: 10-1084-xx
Description: 3"-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-3"-deoxyThymidine,2"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H49N4O8P | M.W.: 744.83 | F.W.: 304.20 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
2’-5’ Linked Oligonucleotides
Cellular DNA and RNA are made up of ribo- and 2’-deoxyribonucleicacids linked together via 3’-5’ phosphodiester linkages and by far comprise thebulk of polynucleic acids found in cells. Much less common are oligonucleotideswhich have 2’-5’ linkages. However, a unique feature of 2’-5’ linkedoligonucleotides is their ability to bind selectively to complementary RNA.These features suggest a number of interesting uses for 2’-5’ linked oligos suchas their use as RNA specific probes or in antisense oligos. Recently, oligoshave been synthesized using 3’-deoxy-2’-phosphoramidites and2’-deoxy-3’-phosphoramidites to produce chimeras with 2’-5’ linked ends and3’-5’ linked central regions. It was found that 2’-5’ phosphorothioate oligos:1) bind selectively to complementary RNA with the same affinity asphosphodiester oligos; 2) exhibit much nonspecific binding to cellular proteins;3) do not activate RNase H. A 3’-deoxynucleoside at the 3’-terminus of anotherwise normal oligonucleotide effectively blocks polymerase extension.
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3"-dT-CE Phosphoramidite
3"-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
3"-dT-CE Phosphoramidite
3"-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1084-02 | 0.25grams | .25grams | 3.36 | 98.67 | 59.2 | 37 | 26.91 | 19.73 | 4.93 |
| 10-1084-90 | 100µmoles | .074grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1084-95 | 50µmoles | .037grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1084-02 | 0.25grams | .25grams | 5.01 | .07 | 93.8 | 58.63 | 42.64 | 5.86 | |
| 10-1084-90 | 100µmoles | .074grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1084-95 | 50µmoles | .037grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1084-02 | 0.25grams | .25grams | 5.01 | .07 | 95.4 | 59.63 | 43.36 | ||
| 10-1084-90 | 100µmoles | .074grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1084-95 | 50µmoles | .037grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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