Oligo Synthesis : CEPs
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3-deaza-dA-CE Phosphoramidite
3-deaza-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1088-02 | 3-deaza-dA-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1088-90 | 3-deaza-dA-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1088-95 | 3-deaza-dA-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
3-deaza-dA-CE Phosphoramidite
3-deaza-dA-CE Phosphoramidite
Glen Research
| 3-deaza-dA-CE Phosphoramidite |
Catalog Number: 10-1088-xx
Description: 3-deaza-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-3-deaza-2"-deoxyAdenosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H53N6O7P | M.W.: 856.95 | F.W.: 312.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Not compatible with mild deprotection at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
STRUCTURE/ACTIVITY RELATIONSHIP
The following products are used to investigate the effect on theactivity of an oligonucleotide when key structural elements are changed. The7-deaza purine monomers lack groups critical for hydrogen bonding.7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G andhave similar electron density. Their presence in oligos is slightly stabilizingrelative to A and G. Unlike G, PPG does not lead to aggregation and G-richoligos can be easily prepared and isolated. 5’-Fluorescein oligos with PPG atthe 5’-terminus are much less quenched than the equivalent G oligos. As a purineanalogue of Thymidine, 7-deaza-2’-deoxyXanthosine (7-deaza-dX) promises to haveinteresting effects on DNA structure of triplexes. 7-Deaza-dX also forms anon-standard base pair with a 2,4-diaminopyrimidine nucleoside analogue.Standard nucleobases have an unshared pair of electrons that project into theminor groove of duplex DNA. Enzymes that interact with DNA, polymerases, reversetranscriptases, restriction enzymes, etc., may use a hydrogen bond donatinggroup to contact the hydrogen bond acceptor in the minor groove.3-Deaza-2’-deoxyadenosine is very interesting in that it maintains the abilityfor regular Watson-Crick hydrogen bonding to T but is lacking the electron pairat the 3-position normally provided by N3.
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3-deaza-dA-CE Phosphoramidite
3-deaza-dA-CE Phosphoramidite
Glen Research
MSDS
Glen Report 17.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
3-deaza-dA-CE Phosphoramidite
3-deaza-dA-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1088-02 | 0.25grams | .25grams | 2.92 | 84 | 50.4 | 31.5 | 22.91 | 16.8 | 4.2 |
| 10-1088-90 | 100µmoles | .086grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1088-95 | 50µmoles | .043grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1088-02 | 0.25grams | .25grams | 4.35 | .07 | 80.6 | 50.38 | 36.64 | 5.04 | |
| 10-1088-90 | 100µmoles | .086grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1088-95 | 50µmoles | .043grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1088-02 | 0.25grams | .25grams | 4.35 | .07 | 82.2 | 51.38 | 37.36 | ||
| 10-1088-90 | 100µmoles | .086grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1088-95 | 50µmoles | .043grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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