Oligo Synthesis : CEPs
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3"-dA-CE Phosphoramidite
3"-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1004-02 | 3"-dA-CE Phosphoramidite | 0.25g | £1,000.00£950.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1004-90 | 3"-dA-CE Phosphoramidite | 100µmoles | £360.00£342.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1004-95 | 3"-dA-CE Phosphoramidite | 50µmoles | £180.00£171.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
3"-dA-CE Phosphoramidite
3"-dA-CE Phosphoramidite
Glen Research
| 3"-dA-CE Phosphoramidite |
Catalog Number: 10-1004-xx
Description: 3"-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-3"-deoxyAdenosine,2"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C47H52N7O7P | M.W.: 857.95 | F.W.: 313.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
BIOLOGICAL MIMICSOur product, 5’-I-dT-CE Phosphoramidite, has been moved to theclick chemistry section of this WEB site and and 2,4-Difluorotoluene (F), as anon-polar mimic of thymidine, has been discontinued. | See Also5’-I-dT in ClickChemistry |
2’-5’ Linked OligonucleotidesCellular DNA and RNA are made up of ribo- and 2’-deoxyribonucleicacids linked together via 3’-5’ phosphodiester linkages and by far comprise thebulk of polynucleic acids found in cells. Much less common are oligonucleotideswhich have 2’-5’ linkages. However, a unique feature of 2’-5’ linkedoligonucleotides is their ability to bind selectively to complementary RNA.These features suggest a number of interesting uses for 2’-5’ linked oligos suchas their use as RNA specific probes or in antisense oligos. Recently, oligoshave been synthesized using 3’-deoxy-2’-phosphoramidites and2’-deoxy-3’-phosphoramidites to produce chimeras with 2’-5’ linked ends and3’-5’ linked central regions. It was found that 2’-5’ phosphorothioate oligos:1) bind selectively to complementary RNA with the same affinity asphosphodiester oligos; 2) exhibit much nonspecific binding to cellular proteins;3) do not activate RNase H. A 3’-deoxynucleoside at the 3’-terminus of anotherwise normal oligonucleotide effectively blocks polymerase extension. |
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3"-dA-CE Phosphoramidite
3"-dA-CE Phosphoramidite
Glen Research
MSDS
Glen Repoort 10.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
3"-dA-CE Phosphoramidite
3"-dA-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1004-02 | 0.25grams | .25grams | 2.91 | 83.67 | 50.2 | 31.38 | 22.82 | 16.73 | 4.18 |
| 10-1004-90 | 100µmoles | .086grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1004-95 | 50µmoles | .043grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1004-02 | 0.25grams | .25grams | 4.35 | .07 | 80.6 | 50.38 | 36.64 | 5.04 | |
| 10-1004-90 | 100µmoles | .086grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1004-95 | 50µmoles | .043grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1004-02 | 0.25grams | .25grams | 4.35 | .07 | 82.2 | 51.38 | 37.36 | ||
| 10-1004-90 | 100µmoles | .086grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1004-95 | 50µmoles | .043grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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