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商品详细Athens Research/Cambio - Excellence in Molecular Biology/100 umole/10-4960-90
Athens Research/Cambio - Excellence in Molecular Biology/100 umole/10-4960-90
Athens Research/Cambio - Excellence in Molecular Biology/100 umole/10-4960-90
商品编号: 10-4960-90
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产品分类: 多肽合成
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • References

3-Cyanovinylcarbazole Phosphoramidite (CNVK)

3-Cyanovinylcarbazole Phosphoramidite (CNVK) An ultrafast photo-crosslinker for DNA and RNA targets

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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10-4960-023-Cyanovinylcarbazole Phosphoramidite (CNVK)0.25g£900.00£855.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-4960-903-Cyanovinylcarbazole Phosphoramidite (CNVK)100 umole£312.00£296.40Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-4960-953-Cyanovinylcarbazole Phosphoramidite (CNVK)50 umoles£160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

3-Cyanovinylcarbazole Phosphoramidite (CNVK)

3-Cyanovinylcarbazole Phosphoramidite (CNVK) An ultrafast photo-crosslinker for DNA and RNA targets

Glen Research

The ability to selectively photo-crosslink a complementary target DNA or RNA strand has a myriad of uses. However, traditional procedures to do so (e.g. psoralen) require long, 20 minute irradiation times with a crosslinking efficiency that is approximately 70%. Furthermore, the wavelength used for photo-reversal of the crosslink is typically 254 nm, which can lead to photoadducts such as a thymidine dimer. Professor Fujimoto from the Japan Advanced Institute of Science and Technology (JAIST) developed a novel photocrosslinker —3-cyanovinylcarbazole nucleoside (CNVK)—that shows a remarkable ability to photo-crosslink pyrimidines of target strands. Irradiation at 366 nm of a duplex containing CNVK opposite thymidine results in 100% cross-linking in 1 second and quantitative crosslinking opposite cytosine in 25 seconds. The photo-reversal of the crosslink was accomplished with irradiation at 312 nm in 3 minutes. This facile reversal reaction is therefore accomplished with no damage to normal DNA. Further research has shown that CNVK can also be cross-linked to an adjacent RNA strand.

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Diluent: Anhydrous Acetonitrile

Coupling: Regular. The use of UltraMILD monomers are preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at Room Temperature in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields. For photo cross-linking conditions please see http://www.glenresearch.com/GlenReports/GR23-14.html.

Storage: Freezer storage, -10 to -30°C, dry in the dark. Light sensitive material.

Stability in Solution: 1-2days

Learn more about the 3-Cyanovinylcarbazole Phosphoramidite (CNVK) in the Glen Report

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3-Cyanovinylcarbazole Phosphoramidite (CNVK)

3-Cyanovinylcarbazole Phosphoramidite (CNVK) An ultrafast photo-crosslinker for DNA and RNA targets

Glen Research

References1. Pieles, U., et al., Nucleic Acids Res, 1989. 17(22): p. 8967-78.2. Douki, Thierry, et al. Journal of Biological Chemistry. 2000 Apr 21;275(16):p. 11678-85; K. Fujimoto, K. Konishi-Hiratsuka, T. Sakamoto, and Y. Yoshimura,ChemBioChem, 2010, 11, 1661-4.3. Y. Yoshimura, and K. Fujimoto, Org Lett, 2008, 10, 3227-30.4. Y. Yoshimura, T. Ohtake, H. Okada, and K. Fujimoto, ChemBioChem, 2009, 10, 1473-6.

For additional information: Glen Report 2011 (1)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。