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商品详细Athens Research/Cambio - Excellence in Molecular Biology/100µmoles/10-1036-90
Athens Research/Cambio - Excellence in Molecular Biology/100µmoles/10-1036-90
Athens Research/Cambio - Excellence in Molecular Biology/100µmoles/10-1036-90
商品编号: 10-1036-90
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产品分类: 多肽合成
公司分类: peptide
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • Protocols
  • Applications & Benefits

2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
10-1036-022-Thio-dT-CE Phosphoramidite0.25g£540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-1036-902-Thio-dT-CE Phosphoramidite100µmoles£236.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-1036-952-Thio-dT-CE Phosphoramidite50µmoles£132.00£125.40Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

2-Thio-dT-CE Phosphoramidite

Structure

Catalog Number: 10-1036-xx

Description: 2-Thio-dT-CE Phosphoramidite

5"-Dimethoxytrityl-(N3/O4-toluoyl)-2"-deoxy-2-thioThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H55N4O8PSM.W.: 879.02F.W.: 320.26

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

The C-nucleoside2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets.2-Aminopurine lacks groups critical for hydrogen bonding and is a mildlyfluorescent base.

Demand for sulfur modified bases continues to expand forinvestigations of oligonucleotide structure, but primarily for cross-linkingpurposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications forphoto cross-linking and photoaffinity labelling experiments. Oligos containing2-thio-dT are useful in examining protein-DNA interaction by acting asphotosensitizing probes. The thiocarbonyl group in 2-thio-dT is especiallyinteresting in that it is available to react with compounds associating with theminor groove of DNA. 2-Amino-A forms a very stable base pair with T containingthree hydrogen bonds but the stability of the base pair with 2-thio-T is greatlydiminished. Due to steric interactions between the 2-thio group of thymidine andthe 2-amino group of 2-amino-A, the base pair contains only a single hydrogenbond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity fornatural oligonucleotides but show little affinity for other similar oligos evenof a complementary sequence.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

MSDS

Glen Report 8.2

Glen Report 11.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

EXTINCTION DATA

ItemNucleosideλMax-1Emax-1λMax-2Emax-2E260
(nm)(ml/µmole)nm(ml/µmole)(ml/µmole)
10-10362-thio-dT27817.522014.810.0

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1036-020.25grams.25grams2.8481.3348.830.522.1816.274.07
10-1036-90100µmoles.088grams120127.55.4541
10-1036-9550µmoles.044grams.53.3321.25.91.67.17
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1036-020.25grams.25grams4.24.0778.44935.644.9
10-1036-90100µmoles.088grams1.5.0723.614.7510.731.48
10-1036-9550µmoles.044grams.75.078.65.383.91.54
Beckman
Cat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nm
Approximate Number of Additions
10-1036-020.25grams.25grams4.24.07805036.36
10-1036-90100µmoles.088grams1.5.0725.215.7511.45
10-1036-9550µmoles.044grams.75.0710.26.384.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。