Oligo Synthesis : CEPs
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2-Thio-dT-CE Phosphoramidite
2-Thio-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1036-02 | 2-Thio-dT-CE Phosphoramidite | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1036-90 | 2-Thio-dT-CE Phosphoramidite | 100µmoles | £236.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1036-95 | 2-Thio-dT-CE Phosphoramidite | 50µmoles | £132.00£125.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2-Thio-dT-CE Phosphoramidite
2-Thio-dT-CE Phosphoramidite
Glen Research
| 2-Thio-dT-CE Phosphoramidite |
Catalog Number: 10-1036-xx
Description: 2-Thio-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-(N3/O4-toluoyl)-2"-deoxy-2-thioThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H55N4O8PS | M.W.: 879.02 | F.W.: 320.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
The C-nucleoside2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets.2-Aminopurine lacks groups critical for hydrogen bonding and is a mildlyfluorescent base.
Demand for sulfur modified bases continues to expand forinvestigations of oligonucleotide structure, but primarily for cross-linkingpurposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications forphoto cross-linking and photoaffinity labelling experiments. Oligos containing2-thio-dT are useful in examining protein-DNA interaction by acting asphotosensitizing probes. The thiocarbonyl group in 2-thio-dT is especiallyinteresting in that it is available to react with compounds associating with theminor groove of DNA. 2-Amino-A forms a very stable base pair with T containingthree hydrogen bonds but the stability of the base pair with 2-thio-T is greatlydiminished. Due to steric interactions between the 2-thio group of thymidine andthe 2-amino group of 2-amino-A, the base pair contains only a single hydrogenbond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity fornatural oligonucleotides but show little affinity for other similar oligos evenof a complementary sequence.
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2-Thio-dT-CE Phosphoramidite
2-Thio-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 8.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2-Thio-dT-CE Phosphoramidite
2-Thio-dT-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1036 | 2-thio-dT | 278 | 17.5 | 220 | 14.8 | 10.0 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1036-02 | 0.25grams | .25grams | 2.84 | 81.33 | 48.8 | 30.5 | 22.18 | 16.27 | 4.07 |
| 10-1036-90 | 100µmoles | .088grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1036-95 | 50µmoles | .044grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1036-02 | 0.25grams | .25grams | 4.24 | .07 | 78.4 | 49 | 35.64 | 4.9 | |
| 10-1036-90 | 100µmoles | .088grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1036-95 | 50µmoles | .044grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1036-02 | 0.25grams | .25grams | 4.24 | .07 | 80 | 50 | 36.36 | ||
| 10-1036-90 | 100µmoles | .088grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1036-95 | 50µmoles | .044grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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