Oligo Synthesis : CEPs
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2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3100-02 | 2"-OMe-A-CE Phosphoramidite | 0.25g | £50.00£47.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3100-05 | 2"-OMe-A-CE Phosphoramidite | 0.5g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3100-10 | 2"-OMe-A-CE Phosphoramidite | 1.0g | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3100-90 | 2"-OMe-A-CE Phosphoramidite | 100µmoles | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
| 2"-OMe-A-CE Phosphoramidite |
2’-OMe-RNA phosphoramidites
Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.
Catalog Number: 10-3100-xx
Description: 2"-OMe-A-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-Adenosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H54N7O8P | M.W.: 887.97 | F.W.: 343.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 3-5 DAYS |
2’-Ome-rna phosphoramidites
Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites aredesigned to produce synthetic oligonucleotides containing nuclease resistant2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of2’-O-methyl oligonucleotides are identical to the procedures foroligodeoxynucleotides.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
MSDS
Glen Report 6.2
Glen Report 6.1
Glen Report 19.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
(1) B. Oberhauser and E. Wagner, Nucleic Acids Res., 1992, 20, 533-538.(2) J. Goodchild, Nucleic Acids Res., 1992, 20, 4607-4612.(3) C. Giovannangeli, N.T. Thuong, and C. Helene, Nucleic Acids Res., 1992, 20, 4275-4281.(4) C. Giovannangeli, M. Rougee, T. Garestier, N.T. Thuong, and C. Helene, Proc. Natl. Acad. Sci. U.S.A., 1992, 89, 8631-8635.(5) J.A. Doudna, J.W. Szostak, A. Rich, and N. Usman, J. Org. Chem., 1990, 55, 5547-5549.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
2"-OMe-A-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-benzoyl-Adenosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H54N7O8P | M.W.: 887.97 | F.W.: 343.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 3-5 DAYS |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA |
| Glen Report 6.1: 2"-OMe-RNA UPDATE |
| Glen Report 19.2: New Lines of Monomers - HT for High Throughput and LC for low cost RN |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 2.82 | 80.67 | 48.4 | 30.25 | 22 | 16.13 | 4.03 |
| 10-3100-05 | 0.5grams | .5grams | 5.63 | 174.33 | 104.6 | 65.38 | 47.55 | 34.87 | 8.72 |
| 10-3100-10 | 1.0gram | 1grams | 11.26 | 362 | 217.2 | 135.75 | 98.73 | 72.4 | 18.1 |
| 10-3100-90 | 100µmoles | .089grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 4.2 | .067 | 77.6 | 48.5 | 35.27 | 4.85 | |
| 10-3100-05 | 0.5grams | .5grams | 8.4 | .067 | 161.6 | 101 | 73.45 | 10.1 | |
| 10-3100-10 | 1.0gram | 1grams | 16.81 | .067 | 329.8 | 206.13 | 149.91 | 20.61 | |
| 10-3100-90 | 100µmoles | .089grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 4.2 | .067 | 79.2 | 49.5 | 36 | ||
| 10-3100-05 | 0.5grams | .5grams | 8.4 | .067 | 163.2 | 102 | 74.18 | ||
| 10-3100-10 | 1.0gram | 1grams | 16.81 | .067 | 331.4 | 207.13 | 150.64 | ||
| 10-3100-90 | 100µmoles | .089grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-A-CE Phosphoramidite
2"-OMe-A-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 2.82 | 80.67 | 48.4 | 30.25 | 22 | 16.13 | 4.03 |
| 10-3100-05 | 0.5grams | .5grams | 5.63 | 174.33 | 104.6 | 65.38 | 47.55 | 34.87 | 8.72 |
| 10-3100-10 | 1.0gram | 1grams | 11.26 | 362 | 217.2 | 135.75 | 98.73 | 72.4 | 18.1 |
| 10-3100-90 | 100µmoles | .089grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 4.2 | .07 | 77.6 | 48.5 | 35.27 | 4.85 | |
| 10-3100-05 | 0.5grams | .5grams | 8.4 | .07 | 161.6 | 101 | 73.45 | 10.1 | |
| 10-3100-10 | 1.0gram | 1grams | 16.81 | .07 | 329.8 | 206.13 | 149.91 | 20.61 | |
| 10-3100-90 | 100µmoles | .089grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3100-02 | 0.25grams | .25grams | 4.2 | .07 | 79.2 | 49.5 | 36 | ||
| 10-3100-05 | 0.5grams | .5grams | 8.4 | .07 | 163.2 | 102 | 74.18 | ||
| 10-3100-10 | 1.0gram | 1grams | 16.81 | .07 | 331.4 | 207.13 | 150.64 | ||
| 10-3100-90 | 100µmoles | .089grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
