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商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.25 grams/10-3151-02

Athens Research/Cambio - Excellence in Molecular Biology/0.25 grams/10-3151-02

商品编号： 10-3151-02

品牌： Athens Research & Technology

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

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产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
10-3151-02	2"-OMe-Ac-C-PACE Phosphoramidite	0.25 grams	£88.00£83.60Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

2"-OMe-A-PACE Phosphoramidite

PACE modifications have enjoyed a resurgence in interest as applied to the field of CRISPR gene editing. In an initial publication, it was shown that single guide RNAs (sgRNA) provided significantly higher activity in cells when 2"-O-methylthiophosphonoacetates were incorporated on the ends of the guide RNA to protect against cellular nucleases.1 In subsequent studies, 2"-OMe PACE modified sgRNAs were also shown to significantly increase on-target specificity of the CRISPR-Cas9 DNA cleavage in eukaryotic cells. In a recent paper, the incorporation of 2"-OMe PACE modified nucleotides in the 20-nucleotide guide region of the sgRNA was shown to decrease off-target cutting by over an order of magnitude while in most cases increasing the overall on-target efficiency as compared to unmodified single guide RNA.2

As an optimal cycle, we recommend using DCI as an activator (30-3150-XX) and a 15 minute coupling time. Following coupling, cap using Unicap with a regular coupling time and then oxidize using 0.5 M CSO for 3 minutes. Alternatively, a 33 minute coupling time using 0.45 M tetrazole, oxidation using low-water iodine (40-4032-XX) followed by capping with 6.5% DMAP as Cap B will give acceptable results. For deprotection, pre-treat the synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes at room temperature to remove 1,1-dimethyl-2-cyanoethyl protecting groups. Rinse the column with ACN, dry under argon and complete deprotection with 40% Methylamine for 2 hours at room temperature.

Formula: C₄₆H₅₈N₅O₁₀P

M.W.: 871.97

F.W.: 361.25

Diluent: Anhydrous Acetonitrile

Coupling: 15 minute coupling time using 0.25M DCI (30-3150-xx) is recommended. Using the phosphoramidite-based capping reagent Unicap (10-4410-xx), cap using a regular coupling time and then oxidize with 0.5M CSO for 3 minutes for best results.

Deprotection: Pretreat synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes at room temperature. Rinse with acetonitrile and dry with argon. Deprotect using 40% methylamine for 2 hours at room temperature.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
				Approximate Number of Additions
Expedite
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	50nm	0.2µm	1µm	15µm
				Approximate Number of Additions

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

SDS

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-OMe-Ac-C-PACE Phosphoramidite

Glen Research

Glen Report 30.1: Significant Improvement of CRISPR Specificity with 2"-OMe-PACE Modifications

Glen Report 30.1: Use of 2"-OMe-PACE Monomers During Oligo Synthesis

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍

Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。

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