Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
2"-OMe-5-F-U-CE Phosphoramidite
2"-OMe-5-F-U-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3132-02 | 2"-OMe-5-F-U-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3132-90 | 2"-OMe-5-F-U-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3132-95 | 2"-OMe-5-F-U-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-OMe-5-F-U-CE Phosphoramidite
2"-OMe-5-F-U-CE Phosphoramidite
Glen Research
| 2"-OMe-5-F-U-CE Phosphoramidite |
Catalog Number: 10-3132-xx
Description: 2"-OMe-5-F-U-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-fluoro-Uridine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H48N4O9PF | M.W.: 778.78 | F.W.: 338.19 |
Diluent: 20% DMF in Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 3-5 days |
minor 2’-Ome-rnaphosphoramidites
To aid in the evaluation of the structures of 2’-OMe-RNAcomplexes, we offer the CE phosphoramidites listed below. 2’-OMe-T is useful intriplex studies while the 2-aminopurine derivative may be tested in ribozymestudies. By supporting an additional hydrogen bond, 2,6-diaminopurine(2-amino-adenosine) binds more strongly with uridine than does adenosine.Oligonucleotides containing 2’-OMe-5-Me-C and 2’-OMe-I would be of interest toresearchers involved in triplex and antisense studies using 2’-OMe-RNA. The usesof 2’-OMe-5-bromo-U phosphoramidite range from crystallographic studies due tothe heavy atom to cross-linking because of its photolability.5-Fluoro-pyrimidine nucleosides have been useful as therapeutic agents and theireffect on the structure and activity of oligonucleotides may be examined usingthe 2’-OMe-RNA derivatives. The 2,4,6-trimethylphenyl (TMP) protected 2’-OMe-Uderivative is a convertible nucleoside and reaction with ammonia leads to the5-fluoro-dC analogue. 2’-OMe-3-deaza-5-aza-C (Reverse C) derivative has thepotential to mimic in oligonucleotides 5-azacytidine, a DNA methylase inhibitor.Its ability to bind as a C will likely be diminished.ABI-style vials aresupplied unless otherwise requested (see note box).
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-5-F-U-CE Phosphoramidite
2"-OMe-5-F-U-CE Phosphoramidite
Glen Research
MSDS
Glen Report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-5-F-U-CE Phosphoramidite
2"-OMe-5-F-U-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3132-02 | 0.25grams | .25grams | 3.21 | 93.67 | 56.2 | 35.13 | 25.55 | 18.73 | 4.68 |
| 10-3132-90 | 100µmoles | .078grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3132-95 | 50µmoles | .039grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3132-02 | 0.25grams | .25grams | 4.79 | .07 | 89.4 | 55.88 | 40.64 | 5.59 | |
| 10-3132-90 | 100µmoles | .078grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3132-95 | 50µmoles | .039grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3132-02 | 0.25grams | .25grams | 4.79 | .07 | 91 | 56.88 | 41.36 | ||
| 10-3132-90 | 100µmoles | .078grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3132-95 | 50µmoles | .039grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
