Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
2"-OMe-5-Br-U-CE Phosphoramidite
2"-OMe-5-Br-U-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3190-02 | 2"-OMe-5-Br-U-CE Phosphoramidite | 0.25g | £692.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3190-90 | 2"-OMe-5-Br-U-CE Phosphoramidite | 100µmoles | £248.00£182.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-OMe-5-Br-U-CE Phosphoramidite
2"-OMe-5-Br-U-CE Phosphoramidite
Glen Research
| 2"-OMe-5-Br-U-CE Phosphoramidite |
Catalog Number: 10-3190-xx
Description: 2"-OMe-5-Br-U-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-bromo-Uridine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H48BrN4O9P | M.W.: 839.72 | F.W.: 399.09 |
Diluent:Acetonitrile:THF (1:1) |
| Coupling: Dissolve in Acetonitrile:THF (1:1).A 6 minute coupling time is recommended. |
| Deprotection: Mild deprotection: Ammonium Hyrdoxide for 24 hours at room temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
minor 2’-Ome-rnaphosphoramidites
To aid in the evaluation of the structures of 2’-OMe-RNAcomplexes, we offer the CE phosphoramidites listed below. 2’-OMe-T is useful intriplex studies while the 2-aminopurine derivative may be tested in ribozymestudies. By supporting an additional hydrogen bond, 2,6-diaminopurine(2-amino-adenosine) binds more strongly with uridine than does adenosine.Oligonucleotides containing 2’-OMe-5-Me-C and 2’-OMe-I would be of interest toresearchers involved in triplex and antisense studies using 2’-OMe-RNA. The usesof 2’-OMe-5-bromo-U phosphoramidite range from crystallographic studies due tothe heavy atom to cross-linking because of its photolability.5-Fluoro-pyrimidine nucleosides have been useful as therapeutic agents and theireffect on the structure and activity of oligonucleotides may be examined usingthe 2’-OMe-RNA derivatives. The 2,4,6-trimethylphenyl (TMP) protected 2’-OMe-Uderivative is a convertible nucleoside and reaction with ammonia leads to the5-fluoro-dC analogue. 2’-OMe-3-deaza-5-aza-C (Reverse C) derivative has thepotential to mimic in oligonucleotides 5-azacytidine, a DNA methylase inhibitor.Its ability to bind as a C will likely be diminished.ABI-style vials aresupplied unless otherwise requested (see note box).
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-5-Br-U-CE Phosphoramidite
2"-OMe-5-Br-U-CE Phosphoramidite
Glen Research
MSDS
Glen Report 11.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-5-Br-U-CE Phosphoramidite
2"-OMe-5-Br-U-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3190-02 | 0.25grams | .25grams | 2.98 | 86 | 51.6 | 32.25 | 23.45 | 17.2 | 4.3 |
| 10-3190-90 | 100µmoles | .084grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3190-02 | 0.25grams | .25grams | 4.44 | .07 | 82.4 | 51.5 | 37.45 | 5.15 | |
| 10-3190-90 | 100µmoles | .084grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3190-02 | 0.25grams | .25grams | 4.44 | .07 | 84 | 52.5 | 38.18 | ||
| 10-3190-90 | 100µmoles | .084grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
