Oligo Synthesis : CEPs
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2"-F-U-CE Phosphoramidite
2"-F-U-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3430-02 | 2"-F-U-CE Phosphoramidite | 0.25g | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3430-05 | 2"-F-U-CE Phosphoramidite | 0.5g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-F-U-CE Phosphoramidite
2"-F-U-CE Phosphoramidite
Glen Research
| 2"-F-U-CE Phosphoramidite |
Catalog Number: 10-3430-xx
Description: 2"-F-U-CE Phosphoramidite
| 5"-Dimethoxytrityl-deoxyUridine, 2"-fluoro-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H46FN4O8P | M.W.: 748.79 | F.W.: 308.16 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. |
| Deprotection:Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature.(Note - heating in AMA will lead to some degradation of the 2"-Fluoro nucleotides.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: A license may be required from Isis Pharmaceuticals, Inc. to incorporate 2"-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 5,670,633; 6,005,087; 6,531,584 and foreign equivalents. |
2’-F-RNA Monomers
2’-Deoxy-2’-fluoro-nucleosides adopt an RNA-type sugarconformation, presumably due to the high electronegativity of fluorine. Becauseof this sugar conformation, RNA duplexes (A-form) are generally morethermodynamically stable than DNA duplexes (B-form). As expected, the additionof 2’-F-RNA residues to oligodeoxynucleotides progressively increases thethermal stability of their duplexes with RNA. The stabilization is additive atapproximately 2° per residue. This compares favorably with 2’-OMe-RNA at around1.5° and RNA at 1.1° per residue. In the meantime, base pair specificity remainsintact.
2’-F-RNA phosphodiester linkages are not nuclease resistant,although the corresponding phosphorothioate linkages are highly resistant.Researchers usually design antisense oligonucleotides to form duplexes with RNA,which are then substrates for RNase H. Uniformly modified 2’-F-RNA/RNA duplexesare not substrates for RNase H. However, it is straightforward to preparechimeric 2’-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhancedbinding to the RNA target, are substrates for RNase H, and are highly nucleaseresistant.
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2"-F-U-CE Phosphoramidite
2"-F-U-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1
Glen Report 17.1
Glen Report 21.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-U-CE Phosphoramidite
2"-F-U-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 3.34 | 98 | 58.8 | 36.75 | 26.73 | 19.6 | 4.9 |
| 10-3430-05 | 0.5grams | .5grams | 6.68 | 209.33 | 125.6 | 78.5 | 57.09 | 41.87 | 10.47 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 4.98 | .07 | 93.2 | 58.25 | 42.36 | 5.83 | |
| 10-3430-05 | 0.5grams | .5grams | 9.97 | .07 | 193 | 120.63 | 87.73 | 12.06 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 4.98 | .07 | 94.8 | 59.25 | 43.09 | ||
| 10-3430-05 | 0.5grams | .5grams | 9.97 | .07 | 194.6 | 121.63 | 88.45 | ||
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