Oligo Synthesis : CEPs
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2-F-dI-CE Phosphoramidite
2-F-dI-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1082-02 | 2-F-dI-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1082-90 | 2-F-dI-CE Phosphoramidite | 100µmoles | £288.00£273.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1082-95 | 2-F-dI-CE Phosphoramidite | 50µmoles | £144.00£136.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2-F-dI-CE Phosphoramidite
2-F-dI-CE Phosphoramidite
Glen Research
| 2-F-dI-CE Phosphoramidite |
Catalog Number: 10-1082-xx
Description: 2-F-dI-CE Phosphoramidite
| 5"-Dimethoxytrityl-2-fluoro-O6-p-nitrophenylethyl-2"-deoxyInosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H53FN7O9P | M.W.: 921.96 | F.W.: varies, 2F=332.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_2-F-dI.pdf). Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
CONVERTIBLE NUCLEOSIDES
The convertible nucleoside strategy is one of the most versatilemethods for producing modifications in bases to examine their effects on DNAstructure and activity. In some cases, with versatility comes difficulty in thatthe convertible base is modified after oligonucleotide synthesis. The chemistryis sometimes complex and base composition analysis of the final oligonucleotideis required to verify structure. The convertible dU monomer can be used tointroduce a variety of modifications at the convertible position, including N, Oand S modifications. Convertible F-dC is by far the simplest approach to thepreparation of oligonucleotides containing F-dC - normal ammonium hydroxidetreatment effects the conversion to F-dC. Convertible dA has been used toprepare oligonucleotides containing multiple points for attachment to solidsupports. In this way, high capacity affinity supports for the purification ofDNA binding proteins have been prepared. 2-F-dI is a convertible nucleoside forthe preparation of 2’-dG derivatives following the displacement of the2-fluorine by primary amines.
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2-F-dI-CE Phosphoramidite
2-F-dI-CE Phosphoramidite
Glen Research
MSDS
Glen Report 15.1
Glen Report 16.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2-F-dI-CE Phosphoramidite
2-F-dI-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1082-02 | 0.25grams | .25grams | 2.71 | 77 | 46.2 | 28.88 | 21 | 15.4 | 3.85 |
| 10-1082-90 | 100µmoles | .092grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1082-95 | 50µmoles | .046grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1082-02 | 0.25grams | .25grams | 4.05 | .07 | 74.6 | 46.63 | 33.91 | 4.66 | |
| 10-1082-90 | 100µmoles | .092grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1082-95 | 50µmoles | .046grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1082-02 | 0.25grams | .25grams | 4.05 | .07 | 76.2 | 47.63 | 34.64 | ||
| 10-1082-90 | 100µmoles | .092grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1082-95 | 50µmoles | .046grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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