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商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3830-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3830-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3830-05
商品编号: 10-3830-05
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产地: 美国(厂家直采)
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产品分类: 多肽合成
公司分类: peptide
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • Protocols
  • References
  • Notes
  • Applications & Benefits

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
10-3800-022"-F-A-ANA CE Phosphoramidite0.25g£400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3800-902"-F-A-ANA CE Phosphoramidite100umole£140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3820-022"-F-G-ANA CE Phosphoramidite0.25g£400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3820-902"-F-G-ANA CE Phosphoramidite100umole£140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3830-022"-F-U-ANA CE Phosphoramidite0.25g£200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3830-052"-F-U-ANA CE Phosphoramidite0.5g£400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

Structure

Catalog Number: 10-3800-xx

Description: 2"-FANA-A-CE Phosphoramidite

5"-Dimethoxytrityl-N6-benzoyl-2"-deoxy-2"-fluoroarabinoadenosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C47H51FN7O7PM.W.: 875.93F.W.: 331.2

Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 1-2 days

2’-F-Arabinonucleic Acid (2’-F-ANA)

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2’ position of the sugar residue. 2’-F-ANA adopts a more DNA-like B-type helix conformation, not through the typical C2’-endo conformation but, rather, through an unusual O4’-endo (east) pucker. However, the presence of the electronegative fluorine leads to a still significant increase (DTm1.2° C/mod) in melting temperature per modification.1 2’-F-ANA-containing oligonucleotides exhibit very high binding specificity to their targets. Indeed, a single mismatch in a 2’-F-ANA – RNA duplex leads to a DTm of -7.2 °C and in a 2’-F-ANA - DNA duplex a DTm of -3.9 °C.2

The presence of fluorine at the 2’ position in 2’-F-ANA leads to increased stability to hydrolysis under basic conditions relative to RNA and even 2’-F-RNA.1,3 The stability of 2’-F-ANA to nucleases also makes this a useful modification for enhancing the stability of oligonucleotides in biological environments.2 2’-F-ANA hybridizes strongly to target RNA and, unlike most 2’ modifications, induces cleavage of the target by RNase H. Phosphorothioate (PS) 2’-F-ANA is routinely used in these applications due to its increased nuclease resistance. Alternating 2’-F-ANA and DNA units provide among the highest potency RNase H-activating oligomers. Both the “altimer” and “gapmer” strand architectures consistently outperform PS-DNA and DNA/RNA gapmers.4

siRNA oligos were found to tolerate the presence of 2’-F-ANA linkages very well. High potency gene silencing was demonstrated5 with siRNA chimeras containing 2’-F-RNA and/or LNA and 2’-F-ANA. The high efficacy of these chimeras was attributed to the combination of the rigid RNA-like properties of 2’-F-RNA and LNA with the DNA-like properties of 2’-F-ANA.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

MSDS

Glen Report 22.2

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

  1. E Viazovkina, M M Mangos, M I Elzagheid, and M H Damha, Curr Protoc Nucleic Acid Chem., 2002, chapter 4, Unit 4 15
  2. J K Watts, and M J Damha, Can J Chem., 2008, 86, 641-656
  3. J K Watts, A Katolik, J Viladoms, and M J Damha, Org Biomol Chem., 2009, 7, 1904-10
  4. A Kalota, et al., Nucleic Acids Res., 2006, 34, 451
  5. G F Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557
  6. J K Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451
  7. T Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675

2"-F-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows:

WO/2009/146556 (siRNA)

WO 03064441 and WO 0220773 (antisense)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

2"-F-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows:

WO/2009/146556 (siRNA)

WO 03064441 and WO 0220773 (antisense)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-ANA CE Phosphoramidites

2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3800-90100µmoles.088grams120127.55.4541
10-3800-020.25grams.25grams2.8581.674930.6322.2716.334.08
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3800-90100µmoles.088grams1.5.0723.614.7510.731.48
10-3800-020.25grams.25grams4.26.0778.849.2535.824.93
Beckman
Cat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nm
Approximate Number of Additions
10-3800-90100µmoles.088grams1.5.0725.215.7511.45
10-3800-020.25grams.25grams4.26.0780.450.2536.55

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。