Oligo Synthesis : CEPs
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2"-F-Ac-C-CE Phosphoramidite
2"-F-Ac-C-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3415-02 | 2"-F-Ac-C-CE Phosphoramidite | 0.25g | £50.00£47.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3415-05 | 2"-F-Ac-C-CE Phosphoramidite | 0.5g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-F-Ac-C-CE Phosphoramidite
2"-F-Ac-C-CE Phosphoramidite
Glen Research
| 2"-F-Ac-C-CE Phosphoramidite |
Catalog Number: 10-3415-xx
Description: 2"-F-Ac-C-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-acetyl-deoxyCytidine,2"-fluoro-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H49FN5O8P | M.W.: 789.84 | F.W.: 307.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. |
| Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2"-Fluoro nucleotides.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: A license may be required from Isis Pharmaceuticals, Inc. to incorporate 2"-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 5,670,633; 6,005,087; 6,531,584 and foreign equivalents. |
2’-F-RNA Monomers
2’-Deoxy-2’-fluoro-nucleosides adopt an RNA-type sugar conformation, presumably due to the high electronegativity of fluorine. Because of this sugar conformation, RNA duplexes (A-form) are generally more thermodynamically stable than DNA duplexes (B-form). As expected, the addition of 2’-F-RNA residues to oligodeoxynucleotides progressively increases the thermal stability of their duplexes with RNA. The stabilization is additive at approximately 2° per residue. This compares favourably with 2’-OMe-RNA at around 1.5° and RNA at 1.1° per residue. In the meantime, base pair specificity remains intact.
2’-F-RNA phosphodiester linkages are not nuclease resistant, although the corresponding phosphorothioate linkages are highly resistant. Researchers usually design antisense oligonucleotides to form duplexes with RNA, which are then substrates for RNase H. Uniformly modified 2’-F-RNA/RNA duplexes are not substrates for RNase H. However, it is straightforward to prepare chimeric 2’-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant.
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2"-F-Ac-C-CE Phosphoramidite
2"-F-Ac-C-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1
Glen Report 17.1
Glen Report 21.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-Ac-C-CE Phosphoramidite
2"-F-Ac-C-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3415-02 | 0.25grams | .25grams | 3.17 | 92.33 | 55.4 | 34.63 | 25.18 | 18.47 | 4.62 |
| 10-3415-05 | 0.5grams | .5grams | 6.33 | 197.67 | 118.6 | 74.13 | 53.91 | 39.53 | 9.88 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3415-02 | 0.25grams | .25grams | 4.72 | .07 | 88 | 55 | 40 | 5.5 | |
| 10-3415-05 | 0.5grams | .5grams | 9.45 | .07 | 182.6 | 114.13 | 83 | 11.41 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3415-02 | 0.25grams | .25grams | 4.72 | .07 | 89.6 | 56 | 40.73 | ||
| 10-3415-05 | 0.5grams | .5grams | 9.45 | .07 | 184.2 | 115.13 | 83.73 | ||
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