当前位置：首页 > 产品中心 > peptide > Athens Research/Cambio - Excellence in Molecular Biology/0.25 g/10-3815-02

商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.25 g/10-3815-02

Athens Research/Cambio - Excellence in Molecular Biology/0.25 g/10-3815-02

商品编号： 10-3815-02

品牌： Athens Research & Technology

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
10-3815-02	2"-F-Ac-C-ANA-CE Phosphoramidite	0.25 g	£200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-3815-05	2"-F-Ac-C-ANA-CE Phosphoramidite	0.5 g	£320.00£304.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue. 2"-F-ANA adopts a more DNA-like β-type helix conformation, not through the typical C2"-endo conformation but, rather, through an unusual O4"-endo (east) pucker. However, the presence of the electronegative fluorine leads to a still significant increase (DTm1.2°C/mod) in melting temperature per modification.¹ 2"-F-ANA-containing oligonucleotides exhibit very high binding specificity to their targets. Indeed, a single mismatch in a 2"-F-ANA - RNA duplex leads to a DTm of -7.2°C and in a 2"-F-ANA - DNA duplex a DTm of -3.9°C.² The presence of fluorine at the 2" position in 2"-F-ANA leads to increased stability to hydrolysis under basic conditions relative to RNA and even 2"-F-RNA.^1,3 The stability of 2"-F-ANA to nucleases also makes this a useful modification for enhancing the stability of oligonucleotides in biological environments.² 2"-F-ANA hybridizes strongly to target RNA and, unlike most 2" modifications, induces cleavage of the target by RNase H. Phosphorothioate (PS) 2"-F-ANA is routinely used in these applications due to its increased nuclease resistance. Alternating 2"-F-ANA and DNA units provide among the highest potency RNase H-activating oligomers. Both the "altimer" and "gapmer" strand architectures consistently outperform PS-DNA and DNA/RNA gapmers.⁴ siRNA oligos were found to tolerate the presence of 2"-F-ANA linkages very well. High potency gene silencing was demonstrated⁵ with siRNA chimeras containing 2"-F-RNA and/or LNA and 2"-F-ANA. The high efficacy of these chimeras was attributed to the combination of the rigid RNA-like properties of 2"-F-RNA and LNA with the DNA-like properties of 2"-F-ANA.

Formula: C_₄_₁H_₄_₉FN_₅O_₈P

M.W.: 789.83

F.W.: 307.17

Diluent: Anhydrous Acetonitrile

Coupling: 6 minute coupling time recommended.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 days

Please Note: 2"-F-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows: WO/2009/146556 (siRNA); WO 03064441 and WO 0220773 (antisense).

E . Viazovkina, M.M. Mangos, M.I. Elzagheid, and M.J. Damha, Curr Protoc Nucleic Acid Chem, 2002, Chapter 4, Unit 4 15.
J.K. Watts, and M.J. Damha, Can. J. Chem., 2008, 86, 641-656.
J.K. Watts, A. Katolik, J. Viladoms, and M.J. Damha, Org Biomol Chem, 2009, 7, 1904-10.
A. Kalota, et al., Nucleic Acids Res., 2006, 34, 451.
G.F. Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557, J.K. Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451, T. Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

MSDS

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

E . Viazovkina, M.M. Mangos, M.I. Elzagheid, and M.J. Damha, Curr Protoc Nucleic Acid Chem, 2002, Chapter 4, Unit 4 15.
J.K. Watts, and M.J. Damha, Can. J. Chem., 2008, 86, 641-656.
J.K. Watts, A. Katolik, J. Viladoms, and M.J. Damha, Org Biomol Chem, 2009, 7, 1904-10.
A. Kalota, et al., Nucleic Acids Res., 2006, 34, 451.
G.F. Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557, J.K. Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451, T. Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Stability note

Synthetic oligonucleotides containing 2"-F-RNA linkages may be deprotected with ammonium hydroxide as normal. Deprotection using AMA at 65°C leads to some degradation and so we recommend the use of AMA at room temperature for 2 hours.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-Ac-C-ANA-CE Phosphoramidite

2"-F-Ac-C-ANA-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-2"-deoxy-2"-fluoroarabinocytidine, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	Approximate Number of Additions
10-3815-02	0.25grams	.25grams	3.17	92.33	55.4	34.63	25.18	18.47	4.62
10-3815-05	0.5grams	.5grams	6.33	197.67	118.6	74.13	53.91	39.53	9.88
Expedite
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Approximate Number of Additions
10-3815-02	0.25grams	.25grams	4.72	.07	88	55	40	5.5
10-3815-05	0.5grams	.5grams	9.45	.07	182.6	114.13	83	11.41
Mermade
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Molarity	30nm	200nm	1000nm
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Approximate Number of Additions
10-3815-02	0.25grams	.25grams	4.72	.07	89.6	56	40.73
10-3815-05	0.5grams	.5grams	9.45	.07	184.2	115.13	83.73

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍

Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。

公司介绍

品牌分类

转铁蛋白和apotranferrins Lab Reagents: Smart Buffers and Reagents 分子生物学用酶：分子生物学用酶蛋白质纯化：阿波罗20毫升超滤免疫球蛋白 qPCR：基因分析和基因面板 PCR : PCR Products 牛奶和血小板蛋白 DNA & RNA Sequencing : Dye Terminator clean-up

Aptamers: Catalogue Aptamers Peptide Synthesis : Amino Acids 运输和其他蛋白质 DNA: Whole DNA STEM学习：STEM工具包低内毒素水平蛋白质提取和纯化-RNA：组织/培养转染-EZ生物系统：细胞系凝血因子分析试剂盒抗体 Gel Electrophoresis: Gel Electrophoresis 中性粒细胞蛋白 Transfection - Biontex: Proteofection 体外毒理学：致突变性试剂盒寡糖合成：CEPs 动物蛋白 Extraction & Purification - DNA: Plasmid 人类蛋白质染色体绘制：人类脂蛋白和载脂蛋白样本拭子：鼻咽拭子酶和抑制剂细胞培养污染控制：检测 Antibodies: Collagenase

显示分类

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.athensresearch.com

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

Related products

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

2"-F-Ac-C-ANA-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA