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商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3850-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3850-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5g/10-3850-05
商品编号: 10-3850-05
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • Protocols
  • References
  • Notes
  • Applications & Benefits

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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10-3850-022"-F-5-Me-U-ANA-CE Phosphoramidite0.25g£200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3850-052"-F-5-Me-U-ANA-CE Phosphoramidite0.5g£400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue. 2"-F-ANA adopts a more DNA-like B-type helix conformation, not through the typical C2"-endo conformation but, rather, through an unusual O4"-endo (east) pucker. However, the presence of the electronegative fluorine leads to a still significant increase (DTm1.2°C/mod) in melting temperature per modification.12"-F-ANA-containing oligonucleotides exhibit very high binding specificity to their targets. Indeed, a single mismatch in a 2"-F-ANA – RNA duplex leads to a DTm of -7.2°C and in a 2"-F-ANA - DNA duplex a DTm of -3.9°C.2

The presence of fluorine at the 2" position in 2"-F-ANA leads to increased stability to hydrolysis under basic conditions relative to RNA and even 2"-F-RNA.1,3The stability of 2"-F-ANA to nucleases also makes this a useful modification for enhancing the stability of oligonucleotides in biological environments.2 2"-F-ANA hybridizes strongly to target RNA and, unlike most 2" modifications, induces cleavage of the target by RNase H. Phosphorothioate (PS) 2"-F-ANA is routinely used in these applications due to its increased nuclease resistance. Alternating 2"-F-ANA and DNA units provide among the highest potency RNase H-activating oligomers. Both the "altimer" and "gapmer" strand architectures consistently outperform PS-DNA and DNA/RNA gapmers.4

siRNA oligos were found to tolerate the presence of 2"-F-ANA linkages very well. High potency gene silencing was demonstrated5 with siRNA chimeras containing 2"-F-RNA and/or LNA and 2"-F-ANA. The high efficacy of these chimeras was attributed to the combination of the rigid RNA-like properties of 2"-F-RNA and LNA with the DNA-like properties of 2"-F-ANA.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Download SDS from Glen website

Glen Report 31.15: New Product - 2′-F-5-Me-U-ANA

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

1. E. Viazovkina, M.M. Mangos, M.I. Elzagheid, and M.J. Damha, Curr Protoc Nucleic Acid Chem, 2002, Chapter 4, Unit 4 15.

2. J.K. Watts, and M.J. Damha, Can. J. Chem., 2008, 86, 641-656.

3. J.K. Watts, A. Katolik, J. Viladoms, and M.J. Damha, Org Biomol Chem, 2009, 7, 1904-10.

4. A. Kalota, et al., Nucleic Acids Res., 2006, 34, 451.

5. G.F. Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557, J.K. Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451, T. Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Intellectual Property

2"-Me-U-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows: WO/2009/146556 (siRNA); WO 03064441 and WO 0220773 (antisense).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2"-F-5-Me-U-ANA-CE Phosphoramidite

Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Usage

Coupling: 6 minute coupling time recommended.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。