Oligo Synthesis : CEPs
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2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3850-02 | 2"-F-5-Me-U-ANA-CE Phosphoramidite | 0.25g | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3850-05 | 2"-F-5-Me-U-ANA-CE Phosphoramidite | 0.5g | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue. 2"-F-ANA adopts a more DNA-like B-type helix conformation, not through the typical C2"-endo conformation but, rather, through an unusual O4"-endo (east) pucker. However, the presence of the electronegative fluorine leads to a still significant increase (DTm1.2°C/mod) in melting temperature per modification.12"-F-ANA-containing oligonucleotides exhibit very high binding specificity to their targets. Indeed, a single mismatch in a 2"-F-ANA – RNA duplex leads to a DTm of -7.2°C and in a 2"-F-ANA - DNA duplex a DTm of -3.9°C.2
The presence of fluorine at the 2" position in 2"-F-ANA leads to increased stability to hydrolysis under basic conditions relative to RNA and even 2"-F-RNA.1,3The stability of 2"-F-ANA to nucleases also makes this a useful modification for enhancing the stability of oligonucleotides in biological environments.2 2"-F-ANA hybridizes strongly to target RNA and, unlike most 2" modifications, induces cleavage of the target by RNase H. Phosphorothioate (PS) 2"-F-ANA is routinely used in these applications due to its increased nuclease resistance. Alternating 2"-F-ANA and DNA units provide among the highest potency RNase H-activating oligomers. Both the "altimer" and "gapmer" strand architectures consistently outperform PS-DNA and DNA/RNA gapmers.4
siRNA oligos were found to tolerate the presence of 2"-F-ANA linkages very well. High potency gene silencing was demonstrated5 with siRNA chimeras containing 2"-F-RNA and/or LNA and 2"-F-ANA. The high efficacy of these chimeras was attributed to the combination of the rigid RNA-like properties of 2"-F-RNA and LNA with the DNA-like properties of 2"-F-ANA.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
Download SDS from Glen website
Glen Report 31.15: New Product - 2′-F-5-Me-U-ANAIf you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
1. E. Viazovkina, M.M. Mangos, M.I. Elzagheid, and M.J. Damha, Curr Protoc Nucleic Acid Chem, 2002, Chapter 4, Unit 4 15.
2. J.K. Watts, and M.J. Damha, Can. J. Chem., 2008, 86, 641-656.
3. J.K. Watts, A. Katolik, J. Viladoms, and M.J. Damha, Org Biomol Chem, 2009, 7, 1904-10.
4. A. Kalota, et al., Nucleic Acids Res., 2006, 34, 451.
5. G.F. Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557, J.K. Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451, T. Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
Intellectual Property
2"-Me-U-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows: WO/2009/146556 (siRNA); WO 03064441 and WO 0220773 (antisense).
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-5-Me-U-ANA-CE Phosphoramidite
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2" position of the sugar residue.5"-Dimethoxytrityl-2"-deoxy-2"-fluoroarabinouridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Research
Usage
Coupling: 6 minute coupling time recommended.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
